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Mineral oil addition as a means of foam control for plant cell cultures in stirred tank fermenters

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Biotechnology Techniques

Summary

The addition of light mineral oil was found to be effective in preventing foaming during plant cell cultivation in stirred tank fermenters without any adverse effect on cell growth. The optimum amount of oil is about 5% (by volume) of the total liquid medium. The best time for the addition is after the lag period of the cell growth cycle, which is about 2 days after cultivation.

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Author notes

  1. Guo-Qing Li

    Present address: Institute of Chemical Metallurgy, Chinese Academy of Sciences, 100080, Beijing, China

  2. Joong Han Shin

    Present address: Dept. of Food Science and Technology, Research Center for New Bio-Materials in Agriculture, Seoul National University, 441-744, Suwon, Korea

Authors and Affiliations

  1. Department of Chemical Engineering, Washington State University, 99164-2710, Pullman, WA, USA

    Guo-Qing Li, Joong Han Shin & James M. Lee

Authors
  1. Guo-Qing Li
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  2. Joong Han Shin
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  3. James M. Lee
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Li, GQ., Shin, J.H. & Lee, J.M. Mineral oil addition as a means of foam control for plant cell cultures in stirred tank fermenters. Biotechnol Tech 9, 713–718 (1995). https://doi.org/10.1007/BF00159235

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  • DOI: https://doi.org/10.1007/BF00159235

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