Abstract
Changing flow rate (F) in arterially perfused cat eyes concomitantly alters the supply of O2 and greatly affects electrical signals: increasing F increases the standing potential (SP) and decreasing F induces the opposite. Corresponding effects on c-wave are more variable.
To separate changes in supply of O2 from changes in F we performed two types of experiments: (1) changing supply of O2 while keeping F constant; this was done by switching to a perfusate of different PO2: and (2) changing F while keeping the supply of O2 constant; this was done by switching to hypoxic or hyperoxic perfusates and adjusting F to keep the b-wave amplitude constant.
Results (1): Decrease in PO2 left the SP essentially unaffected, but increased the c-wave. Increase in PO2 tended to increase the SP and to decrease the c-wave. Results (2): Isolated decrease in F did not affect the SP consistently and left the c-wave unchanged. Isolated increase in F often increased the SP, but also left the c-wave unchanged.
Covariation between SP and amplitude to the c-wave was absent during hyperoxia.
Similar content being viewed by others
References
Gouras P and Hoff M (1970) Retinal function in an isolated perfused mammalian eye. Invest Ophthalmol 9:388–399
Kolder EJW (1983) Slow potentials and microprocessor applications. Doc Ophthalmol Proc Ser 37
Linsenmeier RA and Steinberg RH (1983) Variations of c-wave amplitude in the cat eye. Doc Ophthalmol Proc Ser Vol 37:21–28
Linsenmeier RA, Mines AH and Steinberg RH (1983) Effects of hypoxia and hypercapnia on the light peak and electroretinogram of the cat. Invest Ophthalmol Vis Sci 24:37–46
Linsenmeier RA and Steinberg RH (1984) Mild hypoxia alters K+ homeostasis and pigment epithelial cell membrane responses in cat retina. Invest Ophthalmol Vis Sci 25:3 (Suppl) 289
Niemeyer G (1973) ERG dependence on flow rate in the isolated and perfused mammalian eye. Brain Res 57:203–207
Niemeyer G (1974) O2 dependence of the b-wave in the isolated perfused mammalian eye. Doc Ophthalmol Proc Ser 4:441–448
Niemeyer G (1975) The function of the retina in the perfused eye. Doc Ophthalmol 39:53–116
Niemeyer G (1981) Neurobiology of perfused mammalian eyes. J Neuroscience Meth 3:317–337
Niemeyer G (1983) Light modulation of the standing potential in the perfused mammalian eye: characteristics and responses to acidosis. Doc. Ophthalmol Proc Ser 37: 41–49
Niemeyer G, Nagahara K and Demant E (1982) Effects of changes in the arterial PO2 and PCO2 on the electroretinogram in the cat. Invest Ophthalmol Vis Sci 23:678–683
Nilsson SEG and Skoog KO (1975) Covariation of the simultaneously recorded c-wave and standing potential of the human eye. Acta Ophthalmol 53: 721–730
Papst N, Demant E and Niemeyer G (1982) Changes in PO2 induce retinal autoregulation in vitro. Graefes Arch Clin Exp Ophthalmol 219:6–10
Rodieck RW (1972) Components of the electroretinogram: a reappraisal. Vision Res 12:773–780
Steinberg RH, Linsenmeier RA and Griff ER (1985) Retinal pigment epithelial cell contributions to the electroretinogram and electrooculogram. Prog in Retinal Res. 4:33–66
Zinn KM and Marmor MF (1979) The retinal pigment epithelium. Cambridge, Mass, Harvard University Press
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kreienbühl, B., Niemeyer, G. Standing potential and c-wave during changes in PO2 and flow in the perfused cat eye. Doc Ophthalmol 60, 353–360 (1985). https://doi.org/10.1007/BF00158924
Issue Date:
DOI: https://doi.org/10.1007/BF00158924