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Methanogenic potential activity of mixed liquors: Fluorimetric monitoring

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Summary

Fluorimetric monitoring of potential methanogenic activity (coenzyme F420) of bacterial community from methane digesters is until now resstricted to a few particular cases because severe interference frequnetly occurs, or the concentration is too low when digesting highly diluted wastes. A method of purification and/or concentration, based on purification of the cellular fraction of the sample by centrifugation and washing, is proposed. In biomethanation of spent liquor from citric acid fermentation, a typical case where interference largely obscures the F420 fluorescence signal, precision is shown to be better than ± 5% (confidence interval [t.05 (3)]) for F420 concentration down to 200 nM, and the detection limit is below 10 nM.

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Literature cited

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Authors and Affiliations

  1. Unit of Bioengineering, University of Louvain, B-1348, Louvain-la-Neuve, Belgium

    Binot R. A., H. P. Naveau & E. -J. Nyns

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  1. Binot R. A.
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  2. H. P. Naveau
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  3. E. -J. Nyns
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Binot, R.A., Naveau, H.P. & Nyns, E.J. Methanogenic potential activity of mixed liquors: Fluorimetric monitoring. Biotechnol Lett 3, 623–628 (1981). https://doi.org/10.1007/BF00158690

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  • DOI: https://doi.org/10.1007/BF00158690

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