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Cytological immunodetection of yeast glycoprotein secretion

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Abstract

Expression of antigenic epitopes shared by secreted yeast glycoproteins was studied using specific immunological probes. Application of cytological and ultrastructural methods of immunodetection, employing monoclonal antibodies, permitted us to localize these glycoproteins in the cytoplasm, through the cell wall and at the yeast cell surface. Importance of glycosylation-secretion relationships were evaluated in the secretion process of these molecules. The cell wall crossing and the cell surface distribution of antigenic glycoproteins was described in immunoelectron microscopy and immunofluorescence. Some preferential secretion “ways” were suspected through the yeast cell wall leading to an heterogenous distribution of cell surface glycoproteins destined to be excreted into the medium.

Antigenic variability of cell wall glycoproteins expression was discussed in relation with the glycloprotein secretion.

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References

  1. ArnoldE. and TannerE. (1982): An obligatory role of protein glycosylation in the life cycle of yeast cells -FEBS. Lett. 148: 49–53.

    Google Scholar 

  2. BallouL., AlvaradoE., TsaiP., DellA. and BallouC.E. (1989): Protein glycosylation defects in the Saccharomyces cerevisiae mnn7 mutant class: support for the stop signal proposed for regulation of outer chain elongation - J. Biol. Chem. 264: 11857–11864.

    Google Scholar 

  3. BrawnerD.L. and CutlerJ.E. (1984): Variability in expression of a cell surface determinant on Candida albicans as evidenced by an agglutinating monoclonal antibody - Infect. Immun. 43: 966–972.

    Google Scholar 

  4. BrawnerD.L. and CutlerJ.E. (1986): Ultrastructural and biochemical studies of two dynamically expressed cell surface determinants on Candida albicans - Infect. Immun. 51: 327–336.

    Google Scholar 

  5. BrawnerD.L. and CutlerJ.E. (1986): Variability in expression of cell surface antigens of Candida albicans during morphogenesis - Infect. Immun. 51: 337–343.

    Google Scholar 

  6. BrawnerD.L. and CutlerJ.E. (1987): Cell surface and intracellular expression of two Candida albicans antigens during in vitro and in vivo growth - Microb. Pathog. 2: 249–258.

    Google Scholar 

  7. BrawnerD.L., CutlerJ.E. and BeattyW.L. (1990): Caveats in the investigation of form-specific molecules of Candida albicans - Infect. Immun. 58: 378–383.

    Google Scholar 

  8. CailliezJ.C. and PoulainD. (1988): Analyse cytologique de l'expression d'un épitope porté par les glycoprotéines excrétées par Candida albicans - Ann. Microbiol. Inst. Pasteur 139: 171–188.

    Google Scholar 

  9. CailliezJ.C., BoudrissaA., MackenzieD.W.R. and Poulain,P. (1991): Evaluation of a gold-silver staining method for detection and identification of Candida species by light microscopy - Eur. J. Clin. Microbiol. 9: 886–891.

    Google Scholar 

  10. CasanovaM., GilM.L., CardenosoL., MartinezJ.P. and SentandreuR. (1989): Identification of wall-specific antigens synthesized during germ tube formation by Candida albicans - Infect. Immun. 57: 262–271.

    Google Scholar 

  11. CassoneA., TorosantucciA., BoccaneraM., PellegriniG., PalmaC. and MalvasiF. (1988): Production and characterisation of a monoclonal antibody to a cell-surface glucomannoprotein constituent of Candida albicans and other pathogenic Candida species - J. Med. Microbiol. 27: 233–238.

    Google Scholar 

  12. ChaffinW.L., SkudlarekJ. and MorrowK.J. (1988): Variable expression of surface determinant during proliferation of Candida albicans - Infect. Immun. 56: 302–309.

    Google Scholar 

  13. ChardesT., PiechaczykM., CavaillesV., SalhiS.L., Pau,B. and BastideJ.M. (1986): Production and partial characterization of anti-Candida monoclonal antibodies - Ann. Inst. Pasteur - Immunol. 137: 117–125.

    Google Scholar 

  14. ColeG. (1986): Cell differentiation in conidial fungi -Microbiol. Rev. 50: 95–99.

    Google Scholar 

  15. DouglasL.J. and McCourtieJ. (1983): Effect of tunicamycin treatment on the adherence of Candida albicans to human buccal èpithelial cells. - FEMS Microbiol. Letters 16: 199–202.

    Google Scholar 

  16. DrouhetE. (1988): Overview of fungal antigens. p. 20, In: Fungal antigens. Isolation, Purification and Detection. Drouhet, E., Cole, G.T., De Repentigny, L., Latgé, J.P. and Dupont, B. (ed.) - Plenum Press, New York and London.

    Google Scholar 

  17. Ferro-NovickS., NovickP., FieldC. and ScheckmanR. (1984): Yeast secretory mutants that block the formation of active cell surface enzymes - J. Cell. Biol. 98: 35–43.

    Google Scholar 

  18. Ferro-NovickS., HansenW., SchauerI. and ScheckmanR. (1984): Genes required for completion of import proteins into the endoplasmic reticulum in yeast - J. Cell. Biol. 98: 44–53.

    Google Scholar 

  19. FissE. and BuckleyH.R. (1987): Purification of actin from Candida albicans and comparison with the Candida 48.000-Mr protein - Infect. Immun. 55: 2324–2326.

    Google Scholar 

  20. FruitJ., CailliezJ.C., OddsF.C. and Poulain,D. (1990): Expression of an epitope by surface glycoproteins of Candida albicans. Variability among species, strains and yeast cells of the genus Candida -J. Med. Vet. Mycol. 28: 241–252.

    Google Scholar 

  21. GopalP.K. and Ballou, C.E. (1987): Regulation of the protein glycosylation pathway in yeast: structural control of N-linked oligosaccharide elongation -Proc. Natl. Acad. Sci. USA 84: 8824–8828.

    Google Scholar 

  22. GormanS.P., ScottE.M. and RusselA.D. (1980): Antimicrobial activity, uses and mechanism of action of glutaraldehyde - J. Appl. Bacteriol. 48: 161–190.

    Google Scholar 

  23. HopwoodV., PoulainD., FortierB., EvansG. and Vernes, A. (1986): A monoclonal antibody to a cell wall component of Candida albicans- Infect. Immun. 54: 222–227.

    Google Scholar 

  24. Horisberger M. (1985): The gold method as applied to lectin cytochemistry in transmission and scanning electron microscopy. In: Techniques in immunochemistry. G.R. Bullock and P. Petrusz eds. vol. 3, pp 155–178, Academic Press.

  25. KagayaK., MiyakawaY., FujiharaH., SuzukiM., SoeG. and FukazawaY. (1989): Immunologic significance of diverse specificity of monoclonal antibodies against mannans of Candida albicans - J. Immunol. 143: 3353–3358.

    Google Scholar 

  26. KuoS.C. and LampenO. (1974): Tunicamycin: an inhibitor of yeast glycoprotein synthesis - Biochem. Biophys. Res. Com. 58: 287–295.

    Google Scholar 

  27. LagunasR., DeJuanC. and BegonaB. (1986): Inhibition of biosynthesis of Saccharomyces cerevisiae sugar transport system by tunicamycin - J. Bacteriol. 168: 1484–1486.

    Google Scholar 

  28. Lajean ChaffinW. (1985): Effect of tunicamycin on germ tube and yeast bud formation in Candida albicans - J. Gen. Microbiol. 131: 1853–1861.

    Google Scholar 

  29. LehleL. and TannerW. (1976): The specific site of tunicamycin inhibition in the formation of dolichol-bound N-Acetylglucosamine derivatives - FEBS Lett. 71: 167–170.

    Google Scholar 

  30. LehleL. and SchwarzR.T. (1976): Formation of dolichol monophosphate 2-desoxy-D-glucose and its interference with glycosylation of mannoproteins in yeast - Eur. J. Biochem. 67: 239–245.

    Google Scholar 

  31. LehleL. (1980): Biosynthesis of the core region of yeast mannoproteins. Formation of a glucosylated dolichol-bound oligosaccharide precursor, its transfer to protein and subsequent modification - Eur. J. Biochem. 109: 589–601.

    Google Scholar 

  32. LiR.K. and CutlerJ.E. (1991): A cell surface/plasma membrane antigen of Candida albicans - J. Gen. Microbiol. 137: 455–464.

    Google Scholar 

  33. LinehanL., WadsworthE. and CalderoneR. (1988): Candida albicans C3d receptor, isolated by using a monoclonal antibody - Infect. Immun. 56: 1981–1986.

    Google Scholar 

  34. MiyakawaY., KagayaK., FukazawaY. and SoeG. (1986): Production and characterization of agglutining monoclonal antibodies against predominant antigenic factors for Candida albicans -J. Clin. Microbiol. 23: 881–886.

    Google Scholar 

  35. MizunagaT., IzawaM., IkedaK. and MaruyamaY. (1988): Secretion of an active nonglycosylated form of the repressible acid phosphatase of Saccharomyces cerevisiae in the presence of tunicamycin at low temperatures - J. Biochem. 103: 321–326.

    Google Scholar 

  36. MleczkoJ., LitkeL.L., LarsenH.S. and LaJean ChaffinW. (1989): Effect of glutaraldehyde fixation on cell surface binding capacity of Candida albicans -Infect. Immun. 57: 3247–3249.

    Google Scholar 

  37. MorosoliR., LecherP. and DurandS. (1988): Effect of tunicamycin on xylanase secretion in the yeast Cryptococcus albidus - Arch. Biochem. Biophys. 265: 183–190.

    Google Scholar 

  38. MüllerJ. (1978): Immunological aspects of Candida mycoses, a review of electronmicroscopic studies -Mykosen, Suppl. 1: 289–297.

    Google Scholar 

  39. MüllerJ. (1979): Electronmicroscopy of fungi and fungus diseases. In: Electronmicroscopy in Human Medicine. J.V. Johannessen eds. Mc Graw Hill Book Co, New York.

    Google Scholar 

  40. PolonelliL. and MoraceG. (1986): Specific and common antigenic determinants of Candida albicans isolates detected by monoclonal antibody - J. Clin. Microbiol. 23: 366–368.

    Google Scholar 

  41. PolonelliL. and MoraceG. (1987): Production and characterization of yeast killer toxin monoclonal antibodies - J. Clin. Microbiol. 25: 460–462.

    Google Scholar 

  42. PoulainD., HopwoodV. and VernesA. (1985): Antigenic variability of Candida albicans - C.R.C. Crit. Rev. Microbiol. 12: 223–270.

    Google Scholar 

  43. PoulainD. and PinonJ.M. (1986): Diagnosis of systemic candidiasis: development of cocounterimmunoelectrophoresis- Eur. J. Clin. Microbiol. 5: 420–426.

    Google Scholar 

  44. PoulainD., CailliezJ.C. and DubremetzJ.F. (1989): Secretion of glycoproteins through the cell wall of Candida albicans - Eur. J. Cell Biol. 50: 94–99.

    Google Scholar 

  45. ReissE., KuykendallR. and KaufmanL. (1986): Antigenemia in rabbits infected with Candida albicans serotype B: detection by enzyme immunoassay and preliminary characterization of the antigen - J. Med. Vet. Mycol. 24: 259–269.

    Google Scholar 

  46. ReissE., DeRepentignyL., KuykendallR., CarterA., GalindoR., AugerP., BraggS. and KaufmanL. (1986): Monoclonal antibodies against Candida tropicalis mannan: antigen detection by enzyme immunoassay and immunofluorescence - J. Clin. Microbiol. 24: 796–802.

    Google Scholar 

  47. RotrosenD., EdwardJ.E., GibsonT.R., MooreJ.C., CohenA.M. and GreenI. (1985): Adherence of Candida to cultured vascular endothelial cells: mechanisms of attachment and endothelial cell penetration - J. Infect. Dis. 152: 1264–1274.

    Google Scholar 

  48. Ruiz-HerreraJ. and SentandreuR. (1975): Site of initial glycosylation of mannoproteins from Saccharomyces cerevisiae - J. Bacteriol. 124: 127–133.

    Google Scholar 

  49. San-BiasG. (1982): The cell wall of fungal human pathogens: its possible role in host-parasite relationships - Mycopath. 79: 159–184.

    Google Scholar 

  50. SanchezA., VillanuevaJ.R. and VillaT.G. (1982): Effect of tunicamycin on exo-1,3-beta-D-glucanase synthesis and secretion by cells and protoplasts of Saccharomyces cerevisiae - J. Gen. Microbiol. 128: 3051–3060.

    Google Scholar 

  51. ScheckmanR. (1985): Protein localisation and membrane traffic in yeast - Ann. Rev. Cell Biol. 1: 115–143.

    Google Scholar 

  52. SchrevelJ., GrosD. and MonsignyM. (1981): Cytochemistry of cell glycoconjugates - Progr. Histochem. Cytochem. 14: 1–269.

    Google Scholar 

  53. SmailE. and JonesJ. (1984): Demonstration and solubilization of antigens expressed primarily on the surfaces of Candida albicans germ tubes - Infect. Immun. 45: 74–81.

    Google Scholar 

  54. StrockbineN.A., LargenM. and BuckleyH. (1984): Production and characterization of three monoclonal antibodies to Candida albicans proteins - Infect. Immun. 43: 1012–1018.

    Google Scholar 

  55. SundstromP.M., TamR.M., NicholsE.J. and KennyG.E. (1988): Antigenic differences in the surface mannoproteins of Candida albicans as revealed by monoclonal antibodies - Infect. Immun. 56: 601–606.

    Google Scholar 

  56. TannerW. and LehleL. (1987): Protein glycosylation in yeast - Biochem. Biophys. Acta 906: 81–89.

    Google Scholar 

  57. TojoM, ShibataN., KobayashiM., MikamiT, SuzukiM. and SuzukiS. (1988): Preparation of monoclonal antibodies reactive with beta-1,2-linked oligomannosyl residues in the phosphomannan-protein complex of Candida albicans NIH B-792 strain - Clin. Chem. 34: 539–543.

    Google Scholar 

  58. TrimbleR.B. and MaleyF. (1977). Subunit structure of external invertase from Saccharomyces cerevisiae -J. Biol. Chem. 252: 4409–4412.

    Google Scholar 

  59. TronchinG., BoucharaJ.P., RobertR. and SenetJ.M. (1988): Adherence of Candida albicans germ tubes to plastic: ultrastructural and molecular studies of fibrillar adhesins - Infect. Immun. 56: 1987–1993.

    Google Scholar 

  60. TronchinG., BoucharaJ.P. and RobertR. (1989): Dynamic changes of the cell wall surface of Candida albicans associated with germination and adherence -Eur. J. Cell. Biol. 50: 285–290.

    Google Scholar 

  61. TronchinG., BoucharaJ.P., AnnaixV., RobertR. and SenetJ.M. (1991): Fungal cell adhesion molecules in Candida albicans - Eur. J. Epidemiol. 7: 23–33.

    Google Scholar 

  62. WeinstockG.K. and BallouC.E. (1987): Tunicamycin inhibition of epispore formation in Saccharomyces cerevisiae - J. Bact. 169: 4384–4387.

    Google Scholar 

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Authors and Affiliations

  1. Unité 42 INSERM, Domaine du CERTIA, 369 rue Jules Guesde, 59650, Villeneuve d Ascq, France

    J. C. Cailliez & D. Poulain

  2. Mycological Reference Laboratory, Central Public Health Laboratory, 61 Colindale Avenue, NW9 5HT, London, U.K.

    D. W. R. Mackenzie

  3. Istituto di Microbiologia, Università Degli Studi di Parma, Via A. Gramsci 14, 43100, Parma, Italia

    L. Polonelli

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  1. J. C. Cailliez
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  3. D. W. R. Mackenzie
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  4. L. Polonelli
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Cailliez, J.C., Poulain, D., Mackenzie, D.W.R. et al. Cytological immunodetection of yeast glycoprotein secretion. Eur J Epidemiol 8, 452–459 (1992). https://doi.org/10.1007/BF00158582

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