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Improved differentiation of β-gal+ colonies on X-gal plates containing mycological peptone

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Biotechnology Techniques

Summary

Inclusion of mycological peptone in the base medium of X-gal plates facilitates the detection of isolates of Saccharomyces cerevisiae producing β-galactosidase due to improved colour development. Substitution of tryptone with mycological peptone in a standard Escherichia coli medium (LB) also facilitates colour development on X-gal plates and obviates the need for an inducer. The addition of mycological peptone to the base medium of X-gal plates offers a cost-effective method to improve the detection of the β-galactosidase gene in recombinant strains of S. cerevisiae and E.coli.

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Authors and Affiliations

  1. Department of Microbiology, University College, Galway, Ireland

    Ronan O'Kennedy & John. W. Patching

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  1. Ronan O'Kennedy
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  2. John. W. Patching
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O'Kennedy, R., Patching, J.W. Improved differentiation of β-gal+ colonies on X-gal plates containing mycological peptone. Biotechnol Tech 9, 921–924 (1995). https://doi.org/10.1007/BF00158548

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  • DOI: https://doi.org/10.1007/BF00158548

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