Summary
Aqueous two-phase extraction was employed, as a new approach, for the recovery of amyloglucosidase (AMG) after solid state fermentation. In an aqueous two-phase system of PEG 6000 and potassium phosphate, 95% of the AMG was recovered in the bottom salt phase, with all visible particles from solid fungal bran in the interface, giving a purification factor of 11. Affinity purification of AMG was also done by adsorption onto crosslinked starch, in the presence of PEG or salt. Both were found to enhance the adsorption. In the presence of PEG 6000 (10% w/w), two fold increase in AMG adsorption, 85% of AMG recovery and 6.0 fold of purification were achieved/The purified AMG was found homogeneous by SDS polyacrylamide gel electrophoresis.
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References
Albertsson, P. Å (1971). Partition of cell particles and macromolecules, Almqvist and Wiksell, Stockholm.
Bernfeld, P. (1955). In: Colowick, S. P. and Kaplan, N. O. (Eds.), Methods in Enzymology, Academic Press, New York, Vol 1, pp. 149–58.
Johansson, G. and Joelsson, M. (1985). Enzyme Microb. Technol. 7: 629–634.
Kaul, R. and Mattiasson, B. (1991). In: Extractive Bioconversions, eds. Mattiasson, B. and Holst, O. pp. 173–188. Marcel Dekker, Inc, New York.
Laemmli, U. K. (1970) Nature 227: 680.
Mattiasson, B. (1983). Trends Biotechnol. 1: 16–20.
Miranda, M., Siso, G. I., Gonzalez, P., Murado, M. A. and Miron, J. (1987). Biotechnol. Techniques 1: 195–200.
Nilsson, M., Vijayakumar, A.R., Holst, O., Schornack, C., HÅkanson, H. and Mattiasson, B. (1994). J. Ferment. Bioeng. 78: 356–360.
Pereira, S. S., Torres, E. F., Gonzalez, G.V. and Rojas, M. G. (1993). Appl. Microbiol. Biotechnol. 39: 36–41.
Ramadas, M. and Balasubramaniam, K. (1988). Proc. 44th Annual Session of Sri Lankan Association for the Advancement of Science, Sri Lanka. E2 58 p 220.
Ramadas, M., Holst, O. and Mattiasson, B. (1995). submitted.
Ramesh, M. V. and Lonsane, B. K. (1991) Biotechnol. Letters 13, 355–360.
Smith, P. K., Krohn, R. L., Hermanson, G. T., Mallia, A. K., Gartner, F. H., Provenzano, M. D., Fugimoto, E. K., Goeke, N. M., Olson, B. J. and Klenk, D. C. (1985). Anal. Biochem. 150: 76–85.
Stredansky, M., Kremnicky, L. and Sturdik, E. (1993). Biotechnol. Techniques 7: 69–74
Tengerdy, R. P. (1985). Trends Biotechnol. 3: 96–99.
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Ramadas, M., Holst, O. & Mattiasson, B. Extraction and purification of amyloglucosidase produced by solid state fermentation with Aspergillus niger . Biotechnol Tech 9, 901–906 (1995). https://doi.org/10.1007/BF00158544
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DOI: https://doi.org/10.1007/BF00158544