Isolation of virally-infected insect cells from a population containing infected and uninfected cells
A method was developed that can be used to isolate virally-infected insect cells from a mixed population containing infected and uninfected cells. Specifically, Spodoptera frugiperda Sf-9 cells infected with the Autographa californica multiple nuclear polyhedrosis virus were treated with a primary antibody specific for the gp64 protein present on the surface of virally-infected cells and a secondary antibody labeled with a fluorochrome. The resulting labeled cells were isolated by using fluorescence-activated cell sorting.
KeywordsPrimary Antibody Secondary Antibody Sorting Cell Sorting Insect Cell
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