Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Isolation of virally-infected insect cells from a population containing infected and uninfected cells

  • 15 Accesses

Abstract

A method was developed that can be used to isolate virally-infected insect cells from a mixed population containing infected and uninfected cells. Specifically, Spodoptera frugiperda Sf-9 cells infected with the Autographa californica multiple nuclear polyhedrosis virus were treated with a primary antibody specific for the gp64 protein present on the surface of virally-infected cells and a secondary antibody labeled with a fluorochrome. The resulting labeled cells were isolated by using fluorescence-activated cell sorting.

This is a preview of subscription content, log in to check access.

References

  1. Sommerfelt, M. A. and Sorscher, E. J. (1994) Methods Cell Biol. 42, 563–574.

  2. Domingo, D. L. and Trowbridge, I. S. (1988) J. Biol. Chem. 263, 13386–13392.

  3. Kompier, R. Tramper, J. and Vlak, J. M. (1988) Biotechnol. Lett. 10, 849–854.

  4. Hughes, P. R. and Wood, H. A. (1986) in The Biology of Baculoviruses, Vol. II, (Granados, R. R. and Federici, B. A., Eds.), pp. 1–30, CRC Press, Inc., Boca Raton, Florida.

  5. Leung, D. W., Chen, E. and Goeddel, D. V. (1989) Technique 1, 11–15.

  6. Matschiner, A. (1994) M. S. Thesis, The University of Iowa.

  7. Miller, L. K. (1993) Curr. Opin. Genet. Devel. 3, 97–101.

  8. Morris, T. D. and Miller, L. K. (1993). Virology 197, 339–348.

  9. O'Reilly, D. R., Miller, L. K. and Luckow, V. A. (1992) Baculovirus expression vectors: a laboratory manual. W. H. Freeman and Company, New York.

  10. Pease, E. A., Aust, S. D. and Tien, M. (1991) Biochem. Biophys. Res. Comm. 179, 897–903.

  11. Volkman, L. E. (1986) Curr. Top. Microb. Immunol. 131, 103–118.

  12. Wood, H. A. and Hughes, P. R. (1993) in Advances in Engineered Pesticides (Kim, L., ed.), pp. 261–281, Marcel Dekker, Inc., New York.

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Matschiner, A., Dordick, J.S. & Murhammer, D.W. Isolation of virally-infected insect cells from a population containing infected and uninfected cells. Biotechnol Tech 9, 897–900 (1995). https://doi.org/10.1007/BF00158543

Download citation

Keywords

  • Primary Antibody
  • Secondary Antibody
  • Sorting
  • Cell Sorting
  • Insect Cell