Summary
The immunohistochemical localization of heparan sulphate, collagen type I, III and IV, laminin, tenascin, plasma- and cellular fibronectin was studied in tooth germs from human fetuses. The lamina basalis ameloblastica or membrana preformativa, which separates the pre-ameloblasts from the pre-dentin and dentin, contained heparan sulphate, collagen type IV, laminin and fibronectin. Enamel reacted with antifibronectin, but the reaction varied depending on the type of fibronectin and the source of antibody. In early pre-dentin, collagen type I, laminin, tenascin and fibronectin were present. In late pre-dentin and dentin collagen type I was found in intertubular dentin and in the zone between enamel and dentin. The close relationship between collagen type I in dentin and fibronectin in immature enamel is interesting, as it may contribute to the stabilization of the amelodentinal interface. In dental pulp, collagen type IV and laminin were found in the endothelial basement membranes. Collagen type I and III, tenascin and fibronectin were localized to the mesenchymal intercellular matrix.
The results of this study have supported the assumption that the lamina basalis ameloblastica is a basement membrane, and have lead to the suggestion that ameloblasts are producers of fibronectin or a fibronectin-like substance.
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References
Andersen, H. & Jørgensen, J. B. (1960) Decalcification and staining of archaeological bones, with histochemical interpretation of metachromasia. Stain Technol. 35, 91–6.
Andujar, M. B., Hartmann, D. J., Emonard, H. & Magloire, H. (1988) Distribution and synthesis of type I and type III collagens in developing mouse molar tooth root. Histochemistry 88, 131–40.
Balain, G., Click, E. M. & Bornstein, P. (1980) Location of a collagen-binding domain in fibronectin. J. Biol. Chem. 255, 3234–6.
Borsi, L., Balza, E., Leprini, A., Ponassi, M. & Zardi, L. (1991) Procedure for the purification of the fibronectin proteolytic fragments containing the ED-B oncofetal domain. Analytic. Biochem. 192, 372–9.
Borsi, L., Balza, E., Allemanni, G. & Zardi, L. (1992) Differential expression of the fibronectin isoform containing the ED-B oncofetal domain in normal human fibroblast cell lines originating from different tissues. Exp. Cell Res. 199, 98–105.
Bronckers, A. L. J. J., Lyaruu, D. M. & Wöltgens, J. H. M. (1989) Immunohistochemistry of extracellular matrix proteins during various stages of dentinogenesis. Conn. Tis. Res. 22, 65–70.
Burton-Worster, N., Lust, G. & Wert, R. (1989) Expression of the ED B fibronectin isoform in adult human articular cartilage. Biochem. Biophys. Res. Commun. 165, 782–7.
Dessau, W., Adelmann, B. C. & Timpl, R. (1978) Identification of the sites in collagen α-chains that bind serum anti-gelatin factor (cold-insoluble globulin). Biochem. J. 169, 55–9.
Deutsch, D. (1989) Structure and function of enamel gene products. Anal. Rec. 224, 189–210.
Hørslev-Petersen, K., Kim, K. Y., Pedersen, L. R., Bentsen, K. D., Uldbjerg, N., Oxlund, H., Garbarsch, C., Hahn, E. G., Schuppan, D. & Lorenzen, I. (1988) Serum aminoterminal type III procollagen peptide. Relation to biosynthesis of collagen type III in experimentally induced granulation tissue in rats. APMIS 96, 793–804.
Hurmerinta, K. & Thesleff, I. (1981) Ultrastructure of the epithelial-mesenchymal interface in the mouse tooth germ. J. Craniol. Gen. 1, 191–202.
Ingham, K. C., Brew, S. A. & Isaacs, B. S. (1988) Interaction of fibronectin and its gelatin-binding domains with fluorescent-labeled chains of type I collagen. J. Biol. Chem. 263, 4624–8.
Ishizeki, K., Nagano, H. & Nawa, T. (1990) Features of the aperiodic microfibrils associated with mouse dental basement membrane demonstrated by ultrastructural histochemistry. J. Anat. 173, 139–50.
Katchburian, E. & Burgess, A. M. C. (1983) Lysosomes and removal of the basal lamina of ameloblasts in early stages of odontogenesis. Cell Biol. Intern. Rep. 7, 407–15.
Kubler, M.-D., Lesot, H. & Ruch, J. V. (1988) Temporo-spatial distribution of matrix and microfilament components during odontoblast and ameloblast differentiation. Roux's Arch. Dev. Biol. 197, 212–20.
Laurie, G. W., Leblond, C. P., Inoue, S., Martin, G. R. & Chung, A. (1984) Fine structure of the glomerular basement membrane and immunolocalization of five membrane components to the lamina densa (basal lamina) and its extensions in both glomeruli and tubules of the rat kidney. Am. J. Anat. 169, 463–81.
Lesot, H., Osman, M. & Ruch, J. V. (1981) Immunofluorescent localization of collagens, fibronectin, and laminin during terminal differentiation of odontoblasts. Dev. Biol. 82, 371–81.
Lillie, R. D. & Fullmer, H. M. (1976) Histopathologic Technic and Practical Histochemistry. 4th edn. New York: McGraw-Hill Book Company.
Linde, A., Johansson, S., Jonsson, R. & Jontell, M. (1982) Localization of fibronectin during dentinogenesis in rat incisor. Arch. Oral Biol. 27, 1069–73.
Matthiessen, M. E. & Rømert, P. (1980a) Ultrastructure of the human enamel organ. I. External enamel epithelium, stellate reticulum, and stratum intermedium. Cell Tissue Res. 205, 361–70.
Matthiessen, M. E. & Rømert, P. (1980b) Ultrastructure of the human enamel organ. II. Internal enamel epithelium, preameloblasts, and secretory ameloblasts. Cell Tissue Res. 205, 371–82.
Nanci, A., Ahluwalia, J. P., Pompura, J. R. & Smith, C. E. (1989) Biosynthesis and secretion of enamel proteins in the rat incisor. Anat. Rec. 224, 277–91.
Orams, H. J., Zybert, J. J., Phakey, P. P. & Rachinger, W. A. (1976) Ultrastructural study of human dental enamel using selected-area argon-ion-beam thinning. Arch. Oral Biol. 21, 663–75.
Pearse, A. G. E. (1980) Histochemistry. Theoretical and Applied. Vol 1. Preparative and Optical Technology, 4th edn. Edinburgh: Churchill Livingstone.
Raschkow, I. (1835) Meletemata circa mammalium dentium evolutionem. Inaug.-Dissertation, Bratislaviae (Presburg).
Ruch, J. V. (1985) Session I: Development, form, and function of odontoblasts. Odontoblast differentiation and the formation of the odontoblast layer. J. Dent. Res. 64 (special issue) 489–98.
Schwarzbauer, J. E., Paul, J. I. & Hynes, R. O. (1985) On the origin of species of fibronectin. Proc. Natl Acad. Sci. USA 82, 1424–8.
Takita, K., Ohsaki, Y., Nakata, M. & Kurisu, K. (1987) Immuno-fluorescence localization of type I and type III collagen and fibronectin in mouse dental tissues in late development and during molar eruption. Arch. Oral. Biol. 32, 273–9.
Thesleff, I., Stenman, S., Vaheri, A. & Timpl, R. (1979) Changes in the matrix proteins, fibronectin and collagen, during differentiation of mouse tooth germ. Dev. Biol. 70, 116–26.
Thesleff, I., Barrach, H. J., Foidart, J. M., Vaheri, A., Pratt, R. M. & Martin, G. R. (1981) Changes in the distribution of type IV collagen, laminin, proteoglycan, and fibronectin during mouse tooth development. Dev. Biol. 81, 182–92.
Thesleff, I., Mackie, E., Vainio, S. & Chiquet-Ehrismann, R. (1987) Changes in the distribution of tenascin during tooth development. Development 101, 289–96.
Tung, P. S., Domenicucci, C., Wasi, S. & Sodek, J. (1985) Specific immunohistochemical localization of osteonectin and collagen types I and III in fetal and adult porcine dental tissues. J. Histochem. Cytochem. 33, 531–40.
Vartio, T., Laitinen, L., Närvänen, O., Cutolo, M., Thornell, L.-E., Zardi, L. & Virtanen, I. (1987) Differential expression of the ED sequence-containing form of cellular fibronectin in embryonic and adult human tissues. J. Cell Sci. 88, 419–30.
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Garbarsch, C., Matthiessen, M.E., Olsen, B.E. et al. Immunohistochemistry of the intercellular matrix components and the epithelio-mesenchymal junction of the human tooth germ. Histochem J 26, 110–118 (1994). https://doi.org/10.1007/BF00157959
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DOI: https://doi.org/10.1007/BF00157959