Summary
The autometallographic silver enhancement method is a method for subcellular localization of some heavy metals, such as mercury. However, no quantitative estimate has been made of the amount of mercury demonstrated by the method. In this study, pellets of autometallographic silver grains were prepared from unfixed kidney slices of rats exposed i.p. to mercury chloride containing trace amounts of 203Hg. The slices were silver-enhanced, and subsequently all organic material was removed by enzymatic digestion. During all stages of the experiment the solutions and tissue were gamma-counted. The analysis showed that the final pellets contained approximately 30% of the mercury compared to that found in the slices prior to development and that the mercury was probably located in lysosomes.
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References
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Nørgaard, J.O.R., Ernst, E. & Juhl, S. Efficiency of autometallographic detection of mercury in the rat kidney. Histochem J 26, 100–102 (1994). https://doi.org/10.1007/BF00157957
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DOI: https://doi.org/10.1007/BF00157957