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The initial reaction velocities of lactate dehydrogenase in various cell types

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Summary

The initial reaction velocities (v v ) of lactate dehydrogenase in hepatocytes, cardiac muscle fibres, skeletal (gastrocnemius) muscle fibres, gastric parietal cells, ductal epithelial and acinar cells of the parotid gland, and oocytes were determined, by computer-assisted image analysis, in unfixed sections of these tissues incubated at 37°C on substrate-containing agarose gel films. They were found to fit the equations v i = a1∘A (equation 1) and v i − v = a2∘A (equation 2) reported previously for mouse hepatocytes (Nakae & Stoward, 1993a, b), where v and ∘A are, respectively, the gradients (or steady-state velocities) and the intercepts on the absorbance axis of the linear regression lines of the absorbance (A) of the finalreaction product on incubation times between 1 and 3 min, and a 1 and a 2 are constants. Both equations 1 and 2 fitted the observed v i closely for mouse (a 1 = 2.7, a 2 = 2.2) and human (a 1 = 3.0, a 2 = 1.9) hepatocytes. However, equation 2 fitted the observed v i better than equation 1 for mouse cardiac muscle fibres (a 2 = 1.5), skeletal muscle fibres (a 2 = 1.2), gastric parietal cells (a 2 = 1.7), acinar (a 2 = 1.4) and striated ductal (a 2 = 2.2) epithelial cells of the parotid gland, and oocytes (a 2 = 1.6). The values of v i calculated from the two equations agreed with the observed v i to within about 11%. They ranged from 105 μmole hydrogen equivalents/cm3 cell/min units in hepatocytes to 24 units in parotid acinar cells, but for other cell types they were between 46 and 61 units. These are all considerably higher than values reported previously.

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Authors and Affiliations

  1. Department of Oral Anatomy, School of Dentistry, Tokushima University, 3 Kuramoto-cho, 770, Tokushima, Japan

    Yoshiko Nakae

  2. Department of Anatomy and Physiology, University of Dundee, DD1 4HN, Dundee, Scotland, UK

    Peter J. Stoward

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  1. Yoshiko Nakae
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  2. Peter J. Stoward
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Nakae, Y., Stoward, P.J. The initial reaction velocities of lactate dehydrogenase in various cell types. Histochem J 26, 283–291 (1994). https://doi.org/10.1007/BF00157760

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