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A simple method for the direct extraction of plasmid DNA from yeast

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Biotechnology Techniques

Summary

A rapid and simple method for the small scale isolation of shuttle plasmid DNA from Saccharomyces cerevisiae is described. It uses glass beads to break cells and reagents which are also used in bacterial mini-preps to yield plasmid DNA without chromosomal contamination in sufficient quantities to enable direct visualisation on agarose gels.

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Authors and Affiliations

  1. School of Pure and Applied Biology, University of Wales College of Cardiff, PO Box 915, CF1 3TL, Cardiff, UK

    Michael A. Sobanski & J. Richard Dickinson

Authors
  1. Michael A. Sobanski
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  2. J. Richard Dickinson
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Sobanski, M.A., Dickinson, J.R. A simple method for the direct extraction of plasmid DNA from yeast. Biotechnol Tech 9, 225–230 (1995). https://doi.org/10.1007/BF00157083

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  • DOI: https://doi.org/10.1007/BF00157083

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