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PEG activation and ligand binding for the affinity partitioning of proteins in aqueous two-phase systems

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Biotechnology Techniques


PEG has been activated using epoxy-oxirane, epichlorohydrin and periodate based reactions. The coupling to activated PEG of several protein ligands of different sizes was investigated. Glutathione, trypsin inhibitor, Protein A and anti-BSA have been bound to PEG and used to increase the selectivity of protein separation in aqueous two-phase systems.

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Andrews, B.A., Head, D.M., Dunthorne, P. et al. PEG activation and ligand binding for the affinity partitioning of proteins in aqueous two-phase systems. Biotechnol Tech 4, 49–54 (1990).

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