Summary
A quantitative colorimetric method to measure lignin content in wood has been developed. Red colored complex formed in presence of phloroglucinol-HCl was solubilised by addition of acetic acetyl bromide. Linearity was demonstrated between the absorption values at 545 nm of this complex and 0.5 to 2 mg of lignin of spruce or poplar wood, in presence or absence of ligninolytic fungi.
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References
Adler, E., Björkvist, K.J. and Häggroth, S. (1948) Acta Chim. Scand. 2, 93–94.
Clifford, M.N. (1974). Journal of Chromatography, 94, 321–324.
Effland, M.J. (1977). Tappi, 60, 143–144.
Giger, S. (1985). Ann. Zootech., 34, 85–122.
Hartley, R.D. and Keene, A.S. (1984). Phytochemistry, 23, 1305–1307.
Janshekar, H., Brown, C. and Fiechter, A. (1981). Analytica Chimica Acta. 130, 81–91.
Jellum, M.D. (1960). Agronomy Journal, 52, 54–55.
Johnson, D.V., Moore, W.E. and Zank, L. (1961). Tappi, 44, 793–798.
Kneebone, W.R. (1962). Crop Science, 2, 268.
Morrison, I.M. (1972). J. Sci. Fd. Agric., 23, 455–463.
Wegener, G., Przyklenk, M. and Fengel D. (1983). Holzforschung, 37, 303–307.