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Rapid quantitative determination of DNA in biological processes

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Biotechnology Techniques

Summary

A rapid, easy and reproducible method for the quantitative determination of the DNA (deoxyribonucleic acid) content from unpurified samples is based on fluorescence emitted by DNA-ethidium bromide complex in agarose gel under UV-illumination at a wavelength of 254 nm. No interference is caused by proteins or RNA.

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References

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Authors and Affiliations

  1. GBF, Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300, Braunschweig, F.R.G.

    Riesmeier B. & Kroner K. H.

  2. Department of Chemistry, Helsinki University of Technology, Kemistintie 1, SF-02150, Espoo, Finland

    Nissinen V. & Markkanen P.

  3. Institut für Enzymtechnologie der Universität Düsseldorf, Postfach 2050, D-5170, 1, Jülich, F.R.G.

    Kula M. R.

Authors
  1. Nissinen V.
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  2. Riesmeier B.
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  3. Kroner K. H.
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  4. Markkanen P.
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  5. Kula M. R.
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Nissinen, V., Riesmeier, B., Kroner, K.H. et al. Rapid quantitative determination of DNA in biological processes. Biotechnol Tech 1, 59–62 (1987). https://doi.org/10.1007/BF00156289

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  • DOI: https://doi.org/10.1007/BF00156289

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