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A technique for determining the shear sensitivity of mammalian cells in suspension culture

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Biotechnology Techniques

Summary

A murine hybridoma was subjected to constant shear rates within a couette viscometer for periods of 15 hours. Shear effects on cells were determined by live cell counts, cell viability and by the release of the cytoplasmic enzyme lactate dehydrogenase into the culture medium. Cell damage was observed at a shear rate of 870s-1 but not at 420s-1.

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Queensland, 4067, St. Lucia, QLD, Australia

    Craig G. Smith, Paul F. Greenfield & David H. Randerson

Authors
  1. Craig G. Smith
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  2. Paul F. Greenfield
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  3. David H. Randerson
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Smith, C.G., Greenfield, P.F. & Randerson, D.H. A technique for determining the shear sensitivity of mammalian cells in suspension culture. Biotechnol Tech 1, 39–44 (1987). https://doi.org/10.1007/BF00156285

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  • DOI: https://doi.org/10.1007/BF00156285

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