Skip to main content
SpringerLink
Log in

A simple, efficient, standard-dilution method for cloning hybridomas

  • Published:
Biotechnology Techniques

Summary

A simple standard-dilution method which obviates routine use of viable cell counts, feeder layers and time-consuming scale-up procedures is described. This method can be used to clone monoclonal antibody-producing hybridomas 9–14 days post-fusion. Each cloning cycle takes 5 min. Approximately 60% of the positive, monoclonal antibody-producing hybridomas were successfully cloned and established.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Anonymous. (1980), Hybridoma Techniques. New York: Cold Spring Harbor Laboratory.

  • Galfre, G. and Milstein, C. (1981). Methods in Enzymology, vol. 73. J. T. Langone and H. Van Vunakis, eds. Pp 3–46. New York (London): Academic Press.

    Google Scholar 

  • Ganon, G. and Raymond, Y. (1985). J. Immunol. Meth. 78, 267–270.

    Google Scholar 

  • Oi, V. and Herzenberg, L. (1980). Selected Methods in Cellular Immunology, B. B. Mishell and S. M. Shiigi, eds. Pp. 336–367. San Francisco: W. H. Freeman and Company.

    Google Scholar 

  • Sugasawara, R., Cahoon, B., and Daru, A. (1985). J. Immunol. Meth. 79, 263–275.

    Google Scholar 

  • Van Deusen, R. and Whetstone, C. (1981). Amer. Assn. Vet. Lab. Diag., 24th Annu. Proc., 211–228.

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, Iowa State University, 50011, Ames, Iowa, U.S.A.

    R. L. Mernaugh, C. W. Kaspar & D. P. Durand

  2. Department of Plant Pathology, Seed and Weed Sciences, Iowa State University, 50011, Ames, Iowa, U.S.A.

    J. H. Hill & F. E. Jones

Authors
  1. R. L. Mernaugh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. W. Kaspar
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. P. Durand
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. H. Hill
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. F. E. Jones
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mernaugh, R.L., Kaspar, C.W., Durand, D.P. et al. A simple, efficient, standard-dilution method for cloning hybridomas. Biotechnol Tech 1, 31–33 (1987). https://doi.org/10.1007/BF00156283

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00156283

Keywords

Search

Navigation