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Use of protoplasts in the improvement of filamentous microorganisms. I. Action of N-methyl-N′-nitro-N-nitrosoguanidine on protoplasts of Streptomycetes

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Biotechnology Techniques

Summary

The effects of N-methyl-N−nitro-N-nitroso-guanidine (NTG) on protoplasts of Streptomycetes are markedly different from its action on spores, showing high mutagenic activity even at concentrations having no marked effect on protoplast survival. Strain improvement, eg in chlorotetracycline-producing strains of S. aureofaciens, was most effective when protoplasts were subjected to prolonged treatment (2 h) with low concentrations of NTG (50 /ug/ml).

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Author notes

  1. P. Vyskočil

    Present address: Agrogn Lab., UCF Agrokombinát, 763 15, Slušovice, ČSR

  2. L. Homolka

    Present address: Institute of Microbiology, Czechoslovak Academy of Sciences, Vídeńská 1083, 142 20, 4, Pregue, ČSR

Authors and Affiliations

  1. Research Institute of Antibiotics and Biotransformations, 252 63, Roztoky nr Prague, Czechoslovakia

    P. Vyskočil, L. Homolka & P. Pilát

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  1. P. Vyskočil
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  2. L. Homolka
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  3. P. Pilát
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Vyskočil, P., Homolka, L. & Pilát, P. Use of protoplasts in the improvement of filamentous microorganisms. I. Action of N-methyl-N′-nitro-N-nitrosoguanidine on protoplasts of Streptomycetes. Biotechnol Tech 1, 25–30 (1987). https://doi.org/10.1007/BF00156282

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  • DOI: https://doi.org/10.1007/BF00156282

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