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Determination of malic enzyme activity on permeabilized cells of Saccharomyces cerevisiae using a dissolved CO2 probe

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Biotechnology Techniques

Summary

A method based on the use of a dissolved CO2 probe for yeast malic enzyme activity assay “in situ” is described. This method is well adapted for the estimation of decarboxylating activities of yeast permeabilized cells.

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Authors and Affiliations

  1. Laboratoire de microbiologie et de technologie des fermentations, INRA-IPV, 9,place Viala, 34060, Montpellier, France

    Salmon J. M.

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  1. Salmon J. M.
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Salmon, J.M. Determination of malic enzyme activity on permeabilized cells of Saccharomyces cerevisiae using a dissolved CO2 probe. Biotechnol Tech 1, 7–10 (1987). https://doi.org/10.1007/BF00156278

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  • DOI: https://doi.org/10.1007/BF00156278

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