Abstract
In Chlamydomonas reinhardi, the arg-7 locus is the structural gene for the enzyme argininosuccinate lyase (ASL) which catalyzes the last reaction in arginine biosynthesis
Out of the nine ASL--mutants so far investigated, seven were shown to fall within three distinct groups of complementation.
The activity of ASL produced in diploids formed by complementation is much lower than the activity of ASL in the wild-type strain.
The study of the heat sensitivity of the enzymes seems to indicate that in every diploid only one enzymic variety is formed which in most cases displays a much greater sensitivity to heat than the enzyme formed by the wild-type strain.
These results are discussed in relation to the hypothesis according to which the arg-7 gene in Chlamydomonas reinhardi corresponds to one cistron and the ASL enzyme is composed of at least two identical polypeptide chains.
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Loppes, R., Matagne, R. Allelic complementation between arg-7 mutants in Chlamydomonas reinhardi . Genetica 43, 422–430 (1972). https://doi.org/10.1007/BF00156137
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DOI: https://doi.org/10.1007/BF00156137