Summary
The FPLC system was used for the microdetermination of enzyme activities via the micromeasurement of absorbance of the reaction mixture. The sample loop was directly connected with the monitor. The solution to be measured was injected into the flow of water and the monitor response was measured as a peak height with the built-in integrator. Only 40 μl of the solution are needed for one absorbance measurement.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Lachema (1989). Instructions supplied with the set for the determination of trypsin activity. Lachema, Brno, Czechoslovakia.
Trautschold, I. and Dwenger, A. (1983). In: Methods of Enzymatic Analysis, H.U. Bergmeyer, Ed., Vol. I, pp. 481–527, Verlag Chemie, Weinheim.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Šafařík, I. The use of FPLC for microdetermination of enzyme activity. Biotechnol Tech 5, 485–488 (1991). https://doi.org/10.1007/BF00155500
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00155500