Summary
The use of a rotative flat disc atomizer for the production of biocatalysts immobilized in alginate gel was investigated. The influence of viscosity (0.28 to 1.53 Pa · s), disc diameter (5 to 20 cm), flow rate (0.5 to 2 L/min) and rotational speed (1.6 to 5 rev/s) on drop formation mechanisms, bead diameter and their distributions were investigated. Bead diameters ranging from 1 to 3 mm were been obtained. Flow rate and viscosity increases resulted in higher bead diameters but increasing disc diameter and rotational speed had a decreasing effect. A viscosity of 0.65 Pa · s would prove best for production of beads with good size distribution and mechanical strength. Predictive equations for the mean volumetric bead diameter were determined.
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References
Divies C., Prevost H., and Cavin J.F. (1989). Process 1041, 28–33.
Dombrowski N. (1968). Spray Drying. In: Biochemical and Biological Engineering Science, N. Blakebrough, eds. vol. 2, pp. 209–320, N.Y.: Academic Press.
Dombrowski N., and Lloyd T.L. (1974). Chem. Eng. J. 8, 63–81.
Duteurtre B., Ors P., Charpentier M., and Midoux N. (1986). Patent No. 84 14994, France.
Fair J.R., Steinmeyer D.E., Penny W.R., and Brink J.A. (1973). Liquid-Gas Systems. In: Chemical Engineers' Handbook, 5th ed., R.H. Perry and C.H. Chilton, eds., pp. 58–66, N.Y.: McGraw-Hill Book Co.
Friedman S.J., Gluckert F.A., and Marshall W.R. (1952). Chem. Eng. Prog. 48, 181–191.
Frost A.R. (1981). J. Agric. Eng. Res. 26, 63–78.
Hulst A.C., Tramper J., van't Riet K., and Westerbeek J.M.M. (1985). Biotechnol. Bioeng. 27, 870–876.
Kamiya T., and Kayano A. (1970). Kagaku Kogaku 34, 287–292.
Klein J., and Vorlop K.D., 1982, Immobilized cells. In: ACS Symposium Series, H.W. Blanch, E.T. Papoutsakis, and G. Stephanopoulos, eds., vol. 207, pp. 377–392, Washington D.C.: ACS.
Linko P., and Linko Y.Y. (1984). CRC Crit. Rev. Biotechnol. 1, 289–338.
Marshall W.R. (1954). Atomization and Spray Drying. Chemical Engineering Progress Monograph Series, No. 2, N.Y.: American Institute of Chemical Engineers.
Master K. (1979). Spray Drying Handbook, N.Y.: John Wiley & Sons.
Matsumoto S., Saito K., and Takashima Y. (1974). J. Chem. Eng. Japan 7, 13–19.
Matulovic U., Rasch D., and Wagner F. (1986). Biotechnol. Lett. 8, 485–490.
Morris E.R., Cutler A.N., Ross-Murphy S.B., Rees D.A., and Price J. (1981). Carbohydr. Polym. 1, 5–21.
Rehg T., Dorger C., and Chau P.C. (1986) Biotechnol. Lett. 8, 111–114.
Reis T. (1951). Introduction à la chimiephysique des surfaces, Paris: Dunod.
Smidsrod O., and Haug A. (1972). Acta Chem. Scand. 26, 79–88.
Walton W.H., and Prewett W.C. (1949). Proc. Phys. Soc., 62(6B), 341–350.
Willemot R.M., and Durand G. (1987). Recherche 18, 614–624.
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Bégin, A., Castaigne, F. & Goulet, J. Production of alginate beads by a rotative atomizer. Biotechnol Tech 5, 459–464 (1991). https://doi.org/10.1007/BF00155493
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DOI: https://doi.org/10.1007/BF00155493