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Factors influencing on-line analysis of fermentation alcohols by capillary inlet mass spectrometry

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Biotechnology Techniques

Summary

The two most important factors for rapid, precise analysis of fermentation alcohols by on-line mass spectrometry were the “real” response time of the mass spectrometer and the adsorptive capacity of the tube transporting the off-gas. At this scale (20 L), the bulk liquid mixing time, and the rate of attainment of vapour-liquid equilibrium (VLE) in the fermenter were of lesser importance. No departure from fermenter VLE was observed under extremes of agitation speed, aeration rate, low liquid volume and pH. The total system response time (ts,90%) to a pulse of ethanol injected directly into the fermenter liquid was about 6 minutes.

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Authors and Affiliations

  1. SmithKline Beecham Biologicals, Rue de l'Institut 89, B-1330, Rixensart, Belgium

    J. P. Camelbeeck, D. M. Comberbach, M. Orval, J. O. Pêtre & P. Roelants

  2. Institut Supérieur Industriel Liégeois (I.S.I.L.), Quai Gloesener 6, B-4000, Liège, Belgium

    M. Orval

Authors
  1. J. P. Camelbeeck
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  2. D. M. Comberbach
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  3. M. Orval
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  4. J. O. Pêtre
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  5. P. Roelants
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Camelbeeck, J.P., Comberbach, D.M., Orval, M. et al. Factors influencing on-line analysis of fermentation alcohols by capillary inlet mass spectrometry. Biotechnol Tech 5, 443–448 (1991). https://doi.org/10.1007/BF00155490

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  • DOI: https://doi.org/10.1007/BF00155490

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