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Haemolysis, a tool to evaluate mechanical stress to cells in bioprocess equipment

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Biotechnology Techniques

Summary

Suspensions of fragile sheep erythrocytes were used to compare the levels of mechanical stress in two different crossflow filter units of small pilot scale. The recycling pumps were identified as the critical components mainly responsible for haemolysis. The level of cell damage was largely determined by the pump type and the backpressure applied to increase the transmembrane pressure in the filter.

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References

  • Blackshear, P.R. and Blackshear, G.L. (1987). Mechanical haemolysis. In: Handbook of Bioengineering, R. Skalak and S. Chien, eds., chapt. 15, 15.1–15.19, Mc Graw-Hill.

  • Bronnenmaier, R. and Maerkl, H. (1982). Biotechnol. Bioeng., 24, 553–578.

    Google Scholar 

  • Maiorella, B., Dorin, G., Carion, A. and Harano, D. (1991). Biotechnol. Bioeng., 37, 121–126.

    Google Scholar 

  • Midler, M. and Finn, R.K. (1966). Biotechnol. Bioeng., 8, 71–84.

    Google Scholar 

  • Poncelet, D. and Neufeld, R.J. (1989). Biotechnol. Bioeng., 33, 95–103.

    Google Scholar 

  • Tanaka, S., Yamamoto, S., Yamakoshi, K. and Kamiya, A. (1987). Transact. ASME., 109, 272–278.

    Google Scholar 

  • Williams, A.R. (1973). Biorheology, 10, 303–312.

    Google Scholar 

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Authors and Affiliations

  1. BioSeparation Group, Biotechnology Research Institute, National Research Council of Canada, H4P 2R2, Montreal, Quebec, Canada

    M. Oliver & E. Braendli

Authors
  1. M. Oliver
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  2. E. Braendli
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Oliver, M., Braendli, E. Haemolysis, a tool to evaluate mechanical stress to cells in bioprocess equipment. Biotechnol Tech 5, 405–410 (1991). https://doi.org/10.1007/BF00155483

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  • DOI: https://doi.org/10.1007/BF00155483

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