Skip to main content
SpringerLink
Log in

Stimulation of indirubin production by KNO3 depletion in indole-supplemented suspension culture of Polygonum tinctorium

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

Indole when added at 20 mM to shake flask cultures of Polygonum tinctorium cells on 16th day increased indirubin production from 41 mg/l to 126 mg/l. Use of KNO3 depleted media stimulated indirubin formation up to 88% and 26% in shake flasks and air-lift bioreactors, respectively.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Alfermann, A.W., Bergmann, W., Figur, C., Helmbold, U., Schwantag, D., Schuller, I. and Reinhard, E. (1983). Biotransformation of β-methyldigitoxin to β-methyldigoxin by cell cultures of Digitalis lanata. In: Plant biotechnology, S.H. Mantell and Smith, Eds. pp67–74, Cambridge: Cambridge University Press.

    Google Scholar 

  • Chung, I.S., Kang, Y.M., Oh, J.H., Lee, H.J. and Chae, Y.A. (1994). Biotechnol. Techniques, 8, 789–792.

    Google Scholar 

  • Fox, M. R. and Pierce, J. H. (1990) Textile Chemist and Colorist. 22, 13–15

    Google Scholar 

  • Gamborg, O.L., Miller, R.A. and Ojima, K. (1968). Exp. Cell Res., 50, 148–151.

    Google Scholar 

  • Kim, S.U., Song, K.S., Jung, D.S., Chae, Y.A. and Lee, H.J. (1996). Planta Medica, In press.

  • Hagimori, M., Matsumoto, T. and Obi, Y. (1982). Plant Cell Physiol., 23, 1205–1211.

    Google Scholar 

  • Kohda., H., Niwa, A., Nakamoto, Y. and Takeda, O. (1990). Chemical and Pharmaceutical Bulletin., 38, 523–524.

    Google Scholar 

  • Lee, C.Y. and Kim, W.J. (1990). Natural Spices and Pigments, Seoul: Hyangmoon Publishing Co.

    Google Scholar 

  • Maier, W., Schumann, B. and Groger, D. (1990) Phytochemistry, 29, 817–819.

    Google Scholar 

  • Soh, E.H. and Chae, Y.A. (1993). Korean J. Breed., 25, 133–138.

    Google Scholar 

  • Xia, Z. Q. and Zenk, M. H. (1992). Phytochemistry, 31, 2695–2697.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Genetic Engineering, Kyung Hee University, 449-701, Suwon, Korea

    I. S. Chung, T. Kim & G. W. Bae

  2. RCNBMA, Seoul National University, 441-744, Suwon, Korea

    H. J. Lee & Y. A. Chae

Authors
  1. I. S. Chung
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. T. Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. W. Bae
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. H. J. Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Y. A. Chae
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chung, I.S., Kim, T., Bae, G.W. et al. Stimulation of indirubin production by KNO3 depletion in indole-supplemented suspension culture of Polygonum tinctorium . Biotechnol Lett 18, 947–950 (1996). https://doi.org/10.1007/BF00154627

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00154627

Keywords

Search

Navigation