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Bicarbonate ATP-ase in ciliary body and a theory of Diamox effect on aqueous humor formation

International Ophthalmology volume 1, pages 123–128 (1979)Cite this article

Abstract

Bicarbonate was found to stimulate ATP breakdown by rabbit or cat ciliary body-iris homogenates. Maximum HCO sup−3 stimulation of ATPase with Tris-Hepes buffer occured at pH 8.0. Acid pH and chloride ions in the media reduced the activity of the HCO sup−3 -stimulated ATPase. The Km for ATP was 0.55 mmolar and for HCO sup−3 , 20 mmlar. HCO sup−3 ATPase was not inhibited by acetazolamide added in vitro. It is postulated that ATPase represents the linkage step of energy donor mechanism and active Cl- secretion in acid aqueous humors (human, cat.) or HCO sup−3 secretion in alkaline aqueous humor (rabbit, guinea pig). Inhibition of Cl- or HCO sup−3 secretion by acetazolamide results from decreased intracellular HCO sup−3 levels which, in turn, reduces the stimulation of the HCO sup−3 ATPase.

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  1. Department of Ophthalmology and Visual Science, Yale University School of Medicine, 333 Cedar Street, 06510, New Haven, Conn., USA

    Edward Cotlier

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  1. Edward Cotlier
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Supported by NE1 Grant EY 02013

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Cotlier, E. Bicarbonate ATP-ase in ciliary body and a theory of Diamox effect on aqueous humor formation. Int Ophthalmol 1, 123–128 (1979). https://doi.org/10.1007/BF00154199

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  • DOI: https://doi.org/10.1007/BF00154199

Keywords

  • Bicarbonate ATP-ase