Skip to main content
SpringerLink
Log in

Lyophilization of a 2,4-dimethylphenol-degrading Gram-negative bacterium: Detrimental effect of O2

  • Published:
Biotechnology Techniques

Summary

Survival of a Gram-negative, 2,4-dimethylphenol-degrading bacterium was reduced from 30% to 3% as a result of exposure to O2 for 10 min after lyophilization. Freezing the cells in argon prior to lyophilization increased the survival rate to 100% when cells were not exposed to O2 after lyophilization. Although high survival rates could be achieved, metabolic damage resulted in incomplete metabolization of 2,4-dimethylphenol.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Benedict, R.G., E.S. Sharpe, J. Corman, J.B. Meyers, E.F. Baer, H.H. Hall and R.W. Jackson (1961) Appl. Microbiol. 9, 256–262.

    Google Scholar 

  • Bennet, G.M., A. Seaver and P.H. Calcott (1981) Appl. Environ. Microbiol. 42, 843–849.

    Google Scholar 

  • Dott, W., D. Feidieker, P. Kämpfer, H. Schleibinger, S. Strechel (1989) J. Ind. Microbiol. 4, 365–374.

    Google Scholar 

  • Drews, G. (1983) Mikrobiologisches Praktikum für Naturwissenschaftler. Springer Verlag, Berlin, Heidelberg, New York

    Google Scholar 

  • Heckly, R.J. and R.L. Dimmick (1967) Nature (London), 216, 1003–1004.

    Google Scholar 

  • Poszich-Buscher, C. (1993) Berichte des Forschungszentrums Jülich; 2761.

  • Ray, B., and M.L. Speck (1973) Critical Reviews in Clinical Laboratory Science, 4, 161–213.

    Google Scholar 

  • Volkin, D.B. and A.M. Kibanov (1990) Developments in Biological Standardization, 74, 73–81.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Forschungszentrum Jülich, Institut für Biotechnologie 3, 52425, Jülich, Federal Republic of Germany

    Claudia Eschner, H. Kneifel, L. E. Webb & C. J. Soeder

Authors
  1. Claudia Eschner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Kneifel
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. L. E. Webb
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. J. Soeder
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Eschner, C., Kneifel, H., Webb, L.E. et al. Lyophilization of a 2,4-dimethylphenol-degrading Gram-negative bacterium: Detrimental effect of O2 . Biotechnol Tech 7, 833–836 (1993). https://doi.org/10.1007/BF00153755

Download citation

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00153755

Keywords

Search

Navigation