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Quantitative determination of wood-derived soluble oligosaccharides by HPLC

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Biotechnology Techniques

Abstract

Soluble oligosaccharide carbohydrates from the partial enzymatic hydrolysis of pulp were degraded to monosaccharides by enzymatic hydrolysis using a complete mixture of cellulolytic and hemicellulolytic enzymes and subsequently analysed by HPLC. With this method it was possible to obtain a quantitative estimate of both the 5- and 6-carbon sugars comprising the soluble oligosaccharides.

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Buchert, J., Siika-aho, M., Bailey, M. et al. Quantitative determination of wood-derived soluble oligosaccharides by HPLC. Biotechnol Tech 7, 785–790 (1993). https://doi.org/10.1007/BF00153745

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  • DOI: https://doi.org/10.1007/BF00153745

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