Abstract
Toxicity of an intravitreal injection of gentamicin sulfate, disodium sulbenicillin and cefazolin sodium on the retina was investigated by electroretinogram in albino and pigmented rabbits. Recordings were made before injection and 2 hours and 3, 7, 14, and 21 days after injection.
Significant differences were found in the susceptibility of the electroretinogram components to various antibiotics as follows. Gentamicin 0.24mg/0.1ml irreversibly abolished all the components examined. Sulbenicillin 4.0, 8.0, or 12mg/0.1ml transiently suppressed the fawave and the oscillatory potentials incrementally with increasing dose. Cefazolin 0.5, 2.0, or 5.0mg/0.1 ml selectively reduced the oscillatory potentials, leaving the a- and b-waves almost unattenuated. The cefazolin-suppressed oscillatory potentials recovered within 14 days after injection.
Judging from the most susceptible electroretinogram components to each antibiotic, we recommend intravitreal doses of these antibiotics for clinical use as follows: gentamicin 0.1 mg/0.1 ml, sulbenicillin 2mg/0.1 ml, and cefazolin 0.25mg/0.1 ml.
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References
Bennett T O, Peyman G A (1974) Toxicity of intravitreal aminogylcosides in primates. Can J Ophthalmol 9: 475–478
Conway B P, Campochiaro P A (1986) Macular infarction after endophthalmitis treated with vitrectomy and intravitreal gentamicin. Arch Ophthalmol 104: 367–371
D'Amico D J, Libert J, Kenyon K R, Hanninen L A, Caspers-Velu L (1984) Retinal toxicity of intravitreal gentamicin. Invest Ophthalmol Vis Sei 25: 564–572
D'Amico D J, Caspers-Velu L, Libert J, Shanks E, Schrooyen M, Hanninen L A, Kenyon K R (1985) Comparative toxicity of intravitreal aminoglycoside antibiotics. Am J Ophthalmol 100: 264–275
Fisher J P, Civiletto S E, Forster R K (1982) Toxicity, efficacy and clearance of intravitreally injected cefazolin. Arch Ophthalmol 100: 650–652
Graham R O, Peyman G A, Fishman G (1975) Intravitreal injection of cephaloridine in the treatment of endophthalmitis. Arch Ophthalmol 93: 56–61
Kamei C, Ikegami N, Kiniwa S, Yu Q-H, Miyake H, Tasaka K (1983) Epileptogenic actions of certain cephem antibiotics. Saishin-Igaku 38: 1662–1681
Lawwill T (1972) Practical rabbit electroretinography. Am J Ophthalmol 74; 135–141
Ling C H, Peyman G A, Raichand M (1985) Electron microscopic study of toxicity of intravitreal injections of gentamicin in primates. Can J Ophthalmol 20: 179–183
Palimeris G, Moschos M, Chimonidou E, Panagakis E, Andreanos D, Smirnof T (1978) Intravitreal injection of gentamicin: Experimental findings. Doc Ophthalmol Proc Series 15: 45–52
Peyman G A, May D R, Ericson ES, Apple D (1974) Intraocular injection of gentamicin: Toxic effects and clearance. Arch Ophthalmol 92: 42–47
Peyman G A, Paque J T, Meisels H I, Bennett TO (1975) Postoperative endophthalmitis: A comparison of methods for treatment and prophylaxis with gentamicin. Ophthalmic Surg 6: 45–55
Peyman G A, Raichand M, Bennett TO (1980) Management of endophthalmitis with pars plana vitrectomy. Br J Ophthalmol 64: 472–475
Puliafito C A, Baker A S, Haaf J, Forster C S (1982) Infectious endophthalmitis: Review of 36 cases. Ophthalmology 89: 921–929
Sasovetz D (1978) Ketamine hydrochloride: An effective general anesthetic for use in electroretinography. Ann Ophthalmol 9: 1510–1513
Schenk A G, Peyman G A, Paque J T (1974) The intravitreal use of carbenicillin (Geopen) for treatment of Pseudomonas endophthalmitis. Acta Ophthalmol 52: 707–717
Shibata N, Morita Y, Tanabe J, Kawasaki K, Yonemura D (1973) Effects of change in the osmotic pressure of the incubating medium on the albino rabbit electroretinogram in vitro. Folia Ophthalmol Jpn 24: 539–542
Yonemura D, Kawasaki K, Yanagida T, Tanabe J, Kawaguchi H, Nakata Y (1979) Effects of Ω-amino acids on oscillatory activities of the light-evoked potentials in the retina and visual pathways. Proc 16th ISCEV Symposium, Jpn J Ophthalmol Suppl 339–353
Zachary I G, Forster R K (1976) Experimental intravitreal gentamicin. Am J Ophthalmol 82: 604–611
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Mochizuki, K., Torisaki, M., Kawasaki, K. et al. Retinal toxicity of antibiotics: Evaluation by electroretinogram. Doc Ophthalmol 69, 195–202 (1988). https://doi.org/10.1007/BF00153701
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DOI: https://doi.org/10.1007/BF00153701