Skip to main content
SpringerLink
Log in

Comparative characteristics of chymotrypsin covalently bound on chemically and enzymatically oxidized agaroses

  • Published:
Biotechnology Techniques

Summary

The enzymatically activated agaroses compared with chemically activated show 25 fold lower amount of generated aldehyde groups, 33 fold lower binding capacity for chymotrypsin, 3 fold lower proteolytic as well as amidolytic activity toward AntAlaAlaPheNA of the corresponding fixed enzyme. Trans-cinnamoylimidazole titration data demonstrate 100% active bound enzyme in the case of enzymatically oxidized agaroses and 57% for chemically oxidized. The enzymic activation offers a small number of sites for ligand attachment in a unique microenvironment. The chemical activation yields a suitable matrix for effective chymotrypsin immobilization.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Fleminger,G., Hadas ,E., Wolf ,T., and Solomon ,B. (1990). Appl. Biochem. Biotechnol. 23, 123–137.

    Google Scholar 

  • Petkov,L., Sajdok ,J., Rae ,K., Suchova ,M., Kas ,J., and Turkova ,J. (1990). Biotechnol. Techniques 4, 25–30.

    Google Scholar 

  • Schonbaum,G.R., Zerner ,B.S., and Bender ,M.L. (1961). J. Biol. Chem. 236, 2930–2935.

    Google Scholar 

  • Spackman,D.H., Stein ,W.H., and Moor ,S. (1958). Anal. Chem. 30, 1190–1206.

    Google Scholar 

  • Stambolieva,N., Mateeva, N., Petkov,I., and Yomtova,V. (1989). 5th FECS Int. Conf. on Chem. and Biotechnol. of Biologically Active Natural Products, Varna, Bulgaria, September 1989, 1, 119–123.

  • Stovickova,J., and Turkova ,J. (1988). Biotechnol. Techniques 2, 121–126.

    Google Scholar 

  • Turkova,J., Mikes ,O., Gancev ,K., and Boublik ,M. (1969). Biochim. Biophys. Acta 178, 100–111.

    Google Scholar 

  • Wilchek,M., and Bayer ,E. (1987). Meth. in Enzymol. 138, 429–442.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    Kamen Voivodov & Nikolina Stambolieva

  2. Institute of Organic Chemistry and Biochemistry, Czechoslovak Academy of Sciences, 6, Prague, Czechoslovakia

    Jaroslava Turkova

Authors
  1. Kamen Voivodov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nikolina Stambolieva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Jaroslava Turkova
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Voivodov, K., Stambolieva, N. & Turkova, J. Comparative characteristics of chymotrypsin covalently bound on chemically and enzymatically oxidized agaroses. Biotechnol Tech 5, 219–222 (1991). https://doi.org/10.1007/BF00152785

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00152785

Keywords

Search

Navigation