Skip to main content
SpringerLink
Log in

Alpha-amylase assay with dyed-starch in polyethylene glycol and dextran solutions

  • Published:
Biotechnology Techniques

Summary

The α-amylase assay method with RBB-starch was shown to work well in the presence of polyethylene glycol and dextran, polymers commonly used in aqueous two-phase systems. Neither the polymer type, polymer concentration, nor molecular weight affected the α-amylase activity measurement. On the other hand, the same polymers significantly interfered with the Nelson copper method, the DNS method, and the iodine-starch method.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barman, T. (1969). Enzyme Handbook II, New York: Springer.

    Google Scholar 

  • Bird, R. and Hopkins, R. H. (1954). Biochem. J., 56, 86–99.

    Google Scholar 

  • Whiteside-Carson, V. and Carson, W. W. (1952). Science, 115, 43–43.

    Google Scholar 

  • Hall, F. F., Culp, T. W., Hayakawa, T., Ratliff, C. R., and Hightower, N. C. (1970). Amer. J. Clin. Pathol., 53, 627–634.

    Google Scholar 

  • Ingelman, B. (1948). Acta Chemica. Scan., 2, 803–812.

    Google Scholar 

  • Nelson, N. (1944). J. Biol Chem., 153, 375–380.

    Google Scholar 

  • Noelting, G. and Bernfeld, P. (1948). Helv. Chim. Acta, 31, 286–290.

    Google Scholar 

  • Rinderknecht, H., Wilding, P., and Haverback, B. J. (1967). Experientia, 23, 805–805.

    Google Scholar 

  • Searcy, R. L., Wilding, P., and Berk, J. E. (1967). Clin. Chim. Acta, 15, 189–197.

    Google Scholar 

  • Whistler, R. L. and Louis, C. (1953). Polysaccharide Chemistry, New York: Academic Press.

    Google Scholar 

  • Yoo, Y. J., Hong, J., and Hatch, R. T. (1987). Biotech. Bioeng, 30, 147–151.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Engineering, University of Maryland, 20742, College Park, Maryland, USA

    Kyung Moon Park & Nam Sun Wang

Authors
  1. Kyung Moon Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nam Sun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Park, K.M., Wang, N.S. Alpha-amylase assay with dyed-starch in polyethylene glycol and dextran solutions. Biotechnol Tech 5, 205–208 (1991). https://doi.org/10.1007/BF00152782

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00152782

Keywords

Search

Navigation