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Comparison of an electrochemiluminescent homogenous immunoassay and an ELISA for monitoring productivity in mammalian cell bioreactors

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Biotechnology Techniques

Summary

An Enzyme Linked Immuno Sorbent Assays (ELISA) and an Electro-chemiluminescent Immuno Assay (CIA) are compared for the purpose of monitoring product formation in mammalian cell bioreactors. The ELISA had a relative standard deviation of 10%, compared to 8% for the CIA . The CIA was found to be a fast and accurate alternative to the ELISA. The use of more than one immuno assay format was also shown to provide additional insight into process performance.

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Authors and Affiliations

  1. Biotechnology Process Engineering Center, Massachusetts Institute of Technology, 02139, Cambridge, MA, USA

    Per I. Lindell & Charles L. Cooney

  2. IGEN Inc., 1530 East Jefferson St., 20852, Rockville, MD, USA

    Lee Hall & Tai-Guang Wu

Authors
  1. Per I. Lindell
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  2. Lee Hall
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  3. Tai-Guang Wu
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  4. Charles L. Cooney
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Lindell, P.I., Hall, L., Wu, TG. et al. Comparison of an electrochemiluminescent homogenous immunoassay and an ELISA for monitoring productivity in mammalian cell bioreactors. Biotechnol Tech 5, 187–192 (1991). https://doi.org/10.1007/BF00152779

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  • DOI: https://doi.org/10.1007/BF00152779

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