Summary
The amount of alkali added to the fermentation broth of a recombinant Escherichia coli strain for pH control was monitored on-line by an electronic balance interfaced to a computer. It was successfully correlated with the cell mass, and consequently the cell growth could be well estimated. Using this cell growth estimation technique, an automatic temperature induction was successfully carried out to produce human interleukin-2 in a high yield from E. coli harbouring a temperature controlled expression vector system.
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References
Brandis, J. W., Ditullio, D. F., Lee, J. F. and Arminger, W. B., In Computer Applications in Fermentation technology(N. M. Fish, R. I. Fox and N. F. Thornhill, eds.), SCI, London, 235–251 (1989).
Farrar, W. L., Johnson, H. M. and Farrar, J. J., J. Immunol., 126, 1120–1125 (1981).
Gillis, S., Ferm, M. M., Ou, W. and Smith, K. A., J. Immunol., 120, 2027–2032 (1978).
Gillis, S. and Smith, K. A., Nature, 268, 154–156 (1977).
Gbewonyo, K., Jain, D., Drew, S. W. and Buckland, B. C., Biotechnol. and Bioeng., 34, 234–241 (1989).
Goldberg, A. L. and Goff, S. A.: The selective degradation of abnormal proteins in bacteria, p. 287–314. In Reznikoff, W. and Gold, L. (ed.), Maximizing gene expression, Butterworths, Boston (1986).
Howard, M., Matis, L., Malek, T. R., Shevach, E., Kell, W., Cohen, D., Nakanishi, K. and Paul, W., J. Exp. Med., 158, 2024–2039 (1983).
Kang, S., Kim, S. W., Ha, H. H., Na, D. S., Park, S., Kim, J. Y. and Han, M. H., Korean Biochem. J., 21, 327–333 (1988).
Landwall, P. and Holme, T., J. Gen. Microbiol., 103, 353–358 (1977).
Lee, I. Y., Kim, M. K., Na, D. S., Hahm, K. S., Han, M. H. and Lee, S. B., Kor. J. Appl. Microbiol. Bioeng., 16, 327–333 (1988).
Morgan, D. A., Ruscetti, F. W. and Gallo, R., Science, 193, 1007–1008 (1976).
Mou, D. G. and Cooney, C. L., Biotechnol. Bioeng., 25, 225–255 (1983).
Shaw, J., Monticone, V. and Paetkau, V., J. Immun., 120, 1967–1973 (1978)
Wagner, H., Hardt, C., Heeg, K., Pfizenmaier, K., Solbach, W., Bartlett, R., Stockinger, H. and Rollinghoff, M., Immunol. Rev., 51, 215–255 (1980).
Yun, H. Y., Choi, H. L., Lee, M. K., Kim, S. H., Na, D. S., Lee, S. B., Han, M. H. and Hahm, K. S., Korean Biochem. J., 21, 120–126 (1988).
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Chung, B.H., Seo, D.J., Park, Y.H. et al. Production of recombinant human interleukin-z by Escherichia coli with computer controlled temperature induction. Biotechnol Tech 5, 163–168 (1991). https://doi.org/10.1007/BF00152774
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DOI: https://doi.org/10.1007/BF00152774