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Transformation of Bacillus subtilis by electroporation


Optimal conditions for the transformation of Bacillus subtilis by electroporation were achieved. Frequencies of greater than 105 transformants/μg of plasmid DNA were obtained for a number of strains and plasmids. Increased transformation efficiency of mini-prep DNA from B. subtilis and Escherichia coli was obtained after microdialysis.

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Stephenson, M., Jarrett, P. Transformation of Bacillus subtilis by electroporation. Biotechnol Tech 5, 9–12 (1991).

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  • Escherichia Coli
  • Optimal Condition
  • Bacillus
  • Bacillus Subtilis
  • Transformation Efficiency