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Transformation of Bacillus subtilis by electroporation

Biotechnology Techniques volume 5, pages 9–12 (1991)Cite this article

Summary

Optimal conditions for the transformation of Bacillus subtilis by electroporation were achieved. Frequencies of greater than 105 transformants/μg of plasmid DNA were obtained for a number of strains and plasmids. Increased transformation efficiency of mini-prep DNA from B. subtilis and Escherichia coli was obtained after microdialysis.

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Authors and Affiliations

  1. Institute of Horticultural Research, BN17 6LP, Littlehampton, West Sussex, UK

    Michele Stephenson & Paul Jarrett

Authors
  1. Michele Stephenson
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  2. Paul Jarrett
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Stephenson, M., Jarrett, P. Transformation of Bacillus subtilis by electroporation. Biotechnol Tech 5, 9–12 (1991). https://doi.org/10.1007/BF00152746

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  • DOI: https://doi.org/10.1007/BF00152746

Keywords

  • Escherichia Coli
  • Optimal Condition
  • Bacillus
  • Bacillus Subtilis
  • Transformation Efficiency