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Porin content modifies resistance of Salmonella typhimurium to phagocytosis

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Abstract

Mutants of Salmonella typhimurium, which contain different quantities of outer membrane proteins, show different susceptibility to phagocytosis. We correlate susceptibility to phagocytosis with molecular surface characteristics which are responsible for invasiveness and virulence of bacteria.

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References

  1. Di DonatoA., DraettaG.F., IllianoG., TufanoM.A., SommeseL. and GaldieroF. (1986): Do porins inhibit the macrophage phagocyting activity. - J. Cycl. Nucleot. Prot. Phosphoryl. Res. 11 (2), 87–97.

    Google Scholar 

  2. GalanosC., LuderityO. and WestphalO. (1969): A new method for the extraction of R lipopolysaccharides. -Eur. J. Biochem. 9, 245–249.

    Google Scholar 

  3. GaldieroF., TufanoM.A., RossiF., FilippelliW., DelGaudioF., BerrinoL. and MarmoE. (1987): Effect of Salmonella typhimurium porins on the cardiovascular and renal apparatus. - J. Med. 17 (5/6), 285–298.

    Google Scholar 

  4. GaldieroF., TufanoM.A., SommeseL., FolgoreA. and TedescoF. (1984): Activation of complement system by porins extracted from Salmonella typhimurium. -Infect. Immun. 46, 559–563.

    Google Scholar 

  5. HildebrandtJ.F., MayerL.W., WangS.P. and BuchmanT.M. (1978): Neisseria gonorrhoeae acquire a new principal outer-membrane protein when transformed to resistance to serum bactericidal activity. - Infect. Immun. 20, 267–273.

    Google Scholar 

  6. KuusiN., NurminenM., SaxenH., ValtonenM. and MakelaP.H. (1979): Immunization with major outer membrane proteins in experimental salmonellosis of mice. - Infect. Immun. 25, 857–862.

    Google Scholar 

  7. LaemmliU.K. (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. - Nature (London) 227, 680–685.

    Google Scholar 

  8. LowryO.H., RosebroughN.J., FarrA.L. and RandallR.J. (1951): Protein measurement with Folinphenol reagent. - J. Biol. Chem. 193, 265–275.

    Google Scholar 

  9. Mc BrideH.M., LamberdenP.R., HeckelsJ.E. and WattP.J. (1981): The role of outer membrane proteins in the survival of Neisseria gonorrhoeae P9 within guinea p?g subcutaneous chamber. - J. Gen. Microbiol. 126, 63–67.

    Google Scholar 

  10. MunnC.B., IshiguroE.E., KayW.N. and TrustT.J. (1982): Role of surface components in serum resistance of virulent Aeromonas salmocida. - Infect. Immun. 36, 1069–1075.

    Google Scholar 

  11. NurminenM., LounatmaaK., SarvasM., MakelaP.H. and NakaeT. (1976): Bacteriophage-resistant mutants of Salmonella typhimurium deficient in two major outer membrane proteins. - J. Bacteriol. 127, 941–955.

    Google Scholar 

  12. OsbornJ., GanderJ.E., ParisiE. and CarsonJ. (1972): Mechanism of assembly of the outer membrane of Salmonella typhimurium. - J. Biol. Chem. 247, 3962–3972.

    Google Scholar 

  13. ParsonN.J., KwaasiA.A.A., PereraV.Y., PatelP.V., MartireP.M.V. and SmithH. (1982): Outer membrane proteins of Neisseria gonorrhoeae associated with survival within human polymorphonuclear phagocytes. - J. Gen. Microbiol. 128, 3077–3081.

    Google Scholar 

  14. RosenbergM., GutmickD. and RosenbergE. (1980): Adherence of bacteria to hydrocarbons: a simple method for measuring cell-surface hydrophobicity. -FEMS Microbiol. Lett. 9, 29–33.

    Google Scholar 

  15. TaylorP.W. and PartonR. (1977): A protein factor associated with serum resistance in Escherichia coli. -J. Med. Microbiol. 10, 225–232.

    Google Scholar 

  16. TylewkaS.K., HjertenS. and WadstromT. (1979): Contribution of M protein to the hydrophobic surface properties of Streptococcus pyogenes. -FEMS Microbiol. Lett. 6, 249–253.

    Google Scholar 

  17. TsaiC.M. and FrashC.E. (1982): A sensitive silver stain for detecting lipopolysaccharide in polyacrilamide gels. - Anal. Biochem. 119, 115–119.

    Google Scholar 

  18. TufanoM.A., BerlingieriM.T., SommeseL. and GaldieroF. (1984): Immune response in mice and effects on cells by outer membrane porins from Salmonella typhimurium. - Microbiologica, 7, 353–366.

    Google Scholar 

  19. Tufano M.A., Rossano R. and Galdiero F. (1982): Effect of porins from Salmonella typhimurium on the mouse: immunomodulation and some functional systemic modifications. FEMS Symposium on bacterial and viral inhibition and modulation of host defences. - Pisa, September.

  20. TufanoM.A., SommeseL., CapassoC., FolgoreA., ScafaF. and GaldieroF. (1986): Comparative study of some biological activities of porins extracted from various microorganisms. - Microbiologica 9, 431–442.

    Google Scholar 

  21. VaccaC., LucarelliL., TufanoM.A., GaldieroF. and MarmoE. (1984): Effetti delle porine di Salmonella typhimurium sull'aggregazione di piastrine umane. - Eur. Rev. Med. Pharmacol. Sci. VI, 597–602.

    Google Scholar 

  22. VanOssC.J. (1978): Phagocytosis as a surface phenomenon. - Annal. Rev. Microbiol. 32, 19–39.

    Google Scholar 

  23. VanZwetT.L., ThompsonJ. and VanFurthR. (1975): Effect of glucocortisteroids on the phagocytosis and intracellular killing by peritoneal macrophages. - Infect. Immun. 12, 699–705.

    Google Scholar 

  24. WilkinsonR.G., GemskiP.Jr. and StockerB.A.D. (1972): Non smooth mutants of Salmonella typhimurium: differentiation by phage sensitivity and genetic mapping. - J. Gen. Microbiol. 70, 527–554.

    Google Scholar 

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Tufano, M.A., Sommese, L., Capasso, C. et al. Porin content modifies resistance of Salmonella typhimurium to phagocytosis. Eur J Epidemiol 4, 110–114 (1988). https://doi.org/10.1007/BF00152701

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