Abstract
Forty-four yeasts belonging to the genera Pichia, Candida, Saccharomyces and Kluyveromyces were tested for their potential killer effect on 13 aerobic actinomycetes (6 Nocardia asteroides, 1 N. brasiliensis, 1 N. caviae and 5 Actinomadura madurae). Only a few yeast strains did not display any killer activity against the aerobic actinomycetes studied, thus confirming that the killer phenomenon is widespread among microorganisms. For epidemiological purposes, a killer system was developed. According to their susceptibility to the 9 killer yeasts chosen, it was possible to differentiate the Nocardia and Actinomadura isolates into biotypes. Fitting conditions of the killer system to potential sensitive microorganisms with different characteristics of growth are also discussed.
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GungeN., TamaruA., OzawaF. and SakaguchiK. (1981): Isolation and characterization of linear deoxyribonucleic acid plasmids from Kluyveromyces lactis and the plasmid-associated killer character- J. Bacteriol., 145: 382–390.
MoraceG., ArchibusacciC., SestitoM. and PolonelliL. (1984): Strain differentiation of pathogenic yeasts by the killer system. - Mycopathologia, 84: 81–85.
PierA.C. and FichtnerR.E. (1981): Distribution of serotypes of Nocardia asteroides from animal, human and environmental sources. - J. Clin. Microbiol., 13: 548–553.
PolonelliL., ArchibusacciC., SestitoM. and MoraceG. (1983): Killer system: a simple method for differentiating Candida albicans strains. - J. Clin. Microbiol., 17: 774–780.
PolonelliL., DettoriG., CattelC. and MoraceG. (1987): Biotyping of mycelial fungus cultures by the killer system. - Eur. J. Epidemiol., 3: 237–242.
PolonelliL. and MoraceG. (1986): Reevaluation of the yeast killer phenomenon. - J. Clin. Microbiol., 24: 866–869.
PolonelliL., MoraceG., BarcaioliB.M. and CossuA.L. (1981): Survey of human pathogenic actinomycetes and fungi in soil from Rome and other Italian areas. - Mycopathologia, 73: 161–169.
StevensD.A., PierA.C., BeamanB.L., MorozumiP.A., LovettI.S. and HouangE.T. (1981): Laboratory evaluation of an outbreak of nocardiosis in immunocompromised hosts. - Am. J. Med., 71: 928–934.
WicknerR.B. (1981): Killer system in Saccharomyces cerevisiae. In: J.N. Strathern, E.W. Jones and J.R. Broach (eds), The molecular biology of the yeast Saccharomyces cerevisiae: Life cycle and inheritance. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp. 415–444.
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Morace, G., Dettori, G., Sanguinetti, M. et al. Biotyping of aerobic actinomycetes by modified killer system. Eur J Epidemiol 4, 99–103 (1988). https://doi.org/10.1007/BF00152699
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DOI: https://doi.org/10.1007/BF00152699