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Improvement of the long-term survival of Acetobacterium woodii by limiting acetate production

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Biotechnology Techniques

Summary

The viable count in fructose-grown cultures of Acetobacterium woodii fell to undetectable levels 186 h after the stationary phase was reached. The loss of viability was due to accumulation of undissociated acetic acid, since loss of viability could be induced artificially in cultures grown at pH 6.8 by adjusting the pH to 5.0 or by adding high concentrations of acetate. Survival was greatly improved when cultures were grown at low initial fructose concentrations (≤ 2 g/l) to limit acetate production, since viable cells could still be detected after 73d.

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References

  • Balch, W.E., Schoberth, S., Tanner, R.S. and Wolfe, R.S. (1977). Int. J. Syst. Bacteriol., 27, 355–361.

    Google Scholar 

  • Baronofsky, J.J., Schreurs, W.J.A. and Kashket, E.R. (1984). Appl. Environ. Microbiol., 48, 1134–1139.

    Google Scholar 

  • Davies, E.T. and Stephens, G.M. (1994). In: Advances in Bioprocess Engineering (Galindo, E. and Ramirez,O.T., Eds.), pp. 495 - 499. Dordrecht: Kluwer Academic publishers.

    Google Scholar 

  • Drake, H.L. (1993). In: Microbial Growth on C1 Compounds(Murrell, J.C. and Kelly, D.P., Eds.), pp. 493–507. Andover: Intercept.

    Google Scholar 

  • Eklund, T. (1983). J. Appl. Bacteriol., 54, 383–389.

    Google Scholar 

  • Fuchs, G. (1986). FEMS Microbiol. Rev., 39, 181–213.

    Google Scholar 

  • Godley, A.R., Linnett, P.E. and Robinson, J.P. (1990). Arch. Microbiol., 154, 5–11.

    Google Scholar 

  • Gottschal, J.C. and Morris, J.G. (1981). Biotechnol. Lett., 3, 525–530.

    Google Scholar 

  • Herrero, A.A., Gomez, R.F., Snedcor, B., Tolman, C.J. and Roberts, M.F. (1985). Appl. Microbiol. Biotechnol., 22, 53–62.

    Google Scholar 

  • Hungate, R.E. (1969). In: Methods in Microbiology (Norris, J.R. and Ribbons, D.W., Eds.), Vol. 3B, pp. 117–132. London: Academic Press.

    Google Scholar 

  • Menzel, U. and Gottschalk, G. (1985). Arch. Microbiol., 143, 47–51.

    Google Scholar 

  • Stephens, G.M., Holt, R.A., Gottschal, J.C. and Morris, J.G. (1985). J. Appl. Bacteriol., 59, 597–605.

    Google Scholar 

  • Tschech, A. and Pfennig, N. (1984). Arch. Microbiol., 137, 163–167.

    Google Scholar 

  • Wang, G. and Wang, D.I.C. (1984). Appl. Environ. Microbiol., 47, 294–298.

    Google Scholar 

  • Zeikus, J.G. (1980). Ann. Rev. Microbiol., 34, 423–464.

    Google Scholar 

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Authors and Affiliations

  1. Department of Chemical Engineering, UMIST, PO Box 88, M60 1QD, Manchester, UK

    Mohd. Sahaid Kalil & Gillian M. Stephens

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  1. Mohd. Sahaid Kalil
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  2. Gillian M. Stephens
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Kalil, M.S., Stephens, G.M. Improvement of the long-term survival of Acetobacterium woodii by limiting acetate production. Biotechnol Tech 9, 617–622 (1995). https://doi.org/10.1007/BF00152455

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