Summary
Hydroperoxide lyase converts fatty acid hydroperoxides to oxo-fatty acids which, after further oxidation, are suitable for the synthesis of higher polyamides. An improved method utilizing an acetone powder step for isolation of this enzyme from the unicellular alga Chlorella was developed. Using this procedure a five fold increase in hydroperoxide lyase activity from Chlorella pyrenoidosa was obtained compared to previously used extraction methods. Other Chlorella species were assayed, and it was found that C. fusca also contained significant hydroperoxide lyase activity.
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Nuñez, A., Foglia, T.A. & Piazza, G.J. Improved method for extraction of hydroperoxide lyase from Chorella . Biotechnol Tech 9, 613–616 (1995). https://doi.org/10.1007/BF00152454
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DOI: https://doi.org/10.1007/BF00152454