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Continuous production of tissue plasminogen activator from recombinant CHO cells in a depth filter perfusion system

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Biotechnology Techniques

Summary

A depth filter perfusion system (DFPS), equipped with a 40-μm polypropylene depth filter for cell immobilization, was used for the continuous production of tissue plasminogen activator (t-PA) from recombinant Chinese hamster ovary cells. Final cell density in the DFPS with oxygen control was 1.8×107 cells/mL of the total working volume and maximum t-PA productivity was 2.63 mg/L/day. Dissolved oxygen concentration in the filter matrix was successfully controlled by air sparging and stable operation was possible for more than 20 days.

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Authors and Affiliations

  1. R&D Center, Cheil Foods and Chemicals Inc., 522-1, Tokpyong-Ri, Majang-Myun, Do 467-810, Ichon-Kun, Kyonggi, Korea

    Duk Jae Oh

  2. Department of Chemical Engineering and Bioprocess Engineering Research Center, Korea Advanced Institute of Science and Technology, Daeduk Science Town, 305-701, Taejon, Korea

    Sang Kyo Choi & Ho Nam Chang

Authors
  1. Sang Kyo Choi
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  2. Ho Nam Chang
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  3. Duk Jae Oh
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Choi, S.K., Chang, H.N. & Oh, D.J. Continuous production of tissue plasminogen activator from recombinant CHO cells in a depth filter perfusion system. Biotechnol Tech 9, 567–572 (1995). https://doi.org/10.1007/BF00152445

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  • DOI: https://doi.org/10.1007/BF00152445

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