Biotechnology Techniques

, Volume 7, Issue 9, pp 627–630 | Cite as

Significance of fresh weight to dry cell weight ratio in plant cell suspension cultures

  • In-Suk Park
  • Dong-II Kim


The ratio of fresh weight to dry cell weight (FW/DCW), an index of cell water content, is important in determining the theoretical maximum cell concentration in high density culture of plant cell suspensions. Theoretical maximum cell density for Thalictrum rugosum was estimated to be as high as 137.2 g/l. The FW/DCW values were found to vary from species to species and it was related to medium osmolarity. When the cells were placed in a high osmolarity environment, decrease of FW/DCW was very rapid, taking place within 24 hours.


Cell Density Weight Ratio Suspension Culture Cell Concentration Cell Suspension Culture 
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Copyright information

© Chapman & Hall 1993

Authors and Affiliations

  • In-Suk Park
    • 1
  • Dong-II Kim
    • 1
  1. 1.Department of Biological EngineeringInha UniversityIncheonKorea

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