Significance of fresh weight to dry cell weight ratio in plant cell suspension cultures
- 711 Downloads
The ratio of fresh weight to dry cell weight (FW/DCW), an index of cell water content, is important in determining the theoretical maximum cell concentration in high density culture of plant cell suspensions. Theoretical maximum cell density for Thalictrum rugosum was estimated to be as high as 137.2 g/l. The FW/DCW values were found to vary from species to species and it was related to medium osmolarity. When the cells were placed in a high osmolarity environment, decrease of FW/DCW was very rapid, taking place within 24 hours.
KeywordsCell Density Weight Ratio Suspension Culture Cell Concentration Cell Suspension Culture
Unable to display preview. Download preview PDF.
- Battat, E., Rokem, J.S., and Goldberg, I. (1989). Plant Cell Reports 7, 652–654.Google Scholar
- Drapeau, D., Blanch, H.W., and Wilke, C.R. (1986). Biotechnol. Bioeng. 28, 1555–1563.Google Scholar
- Kim, D.-I., Pedersen, H., and Chin, C.-K. (1990). J. Biotechnol. 16, 297–304.Google Scholar
- Kim, D.-I., Pedersen, H., and Chin, C.-K. (1991a). Biotechnol. Bioeng. 38, 331–339.Google Scholar
- Kim, D.-I., Pedersen, H., and Chin, C.-K. (1991b). Appl. Microbiol. Biotechnol. 34, 726–729.Google Scholar
- Knobloch, K.-H. and Berlin, J. (1980). Z Naturforsch. bd35c, 551–556.Google Scholar
- Lipsky, A.K. (1992). J. Biotechnol., 26, 83–97.Google Scholar
- Mantell, S.H., and Smith, H. (1983). Cultural factors that influence secondary metabolite accumulations in plant cell and tissue cultures. In: Plant Biotechnology, S.H. Mantell and H. Smith, eds., pp. 75–108, Cambridge: Cambridge University Press.Google Scholar
- Merillon, J.M., Rideau, M., and Chenieux, J.C. (1984). Planta Medica 50, 497–501.Google Scholar
- Panda, A.K., Mishira, S., and Bisaria, V.S. (1992). Biotechnol. Bioeng. 39, 1043–1051.Google Scholar
- Zhong, J.-J., Seki, T., Kinoshita, S.-I., and Yoshida, T. (1992). Biotechnol. Bioeng. 40, 1256–1262.Google Scholar