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Monitoring of the response of elderly individuals to pneumococcal vaccination with the aid of a novel ELISA

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Abstract

An ELISA for assessing pneumococcal anticapsular antibodies in human sera is described. Sensitization was carried out by adding to avidin-coated polystyrene plates biotinylated capsular polyosides. This ELISA was used for the determination of anticapsular antibody levels in pre- and post-immunization sera from 20 elderly individuals. The anticapsular antibody titres differed significantly in sera taken prior to and after vaccination. Eighteen of the 20 sera tested showed mean fold increases of the antibody level greater than 2 (2.1 to 21.0) as a result of vaccination. Since morbidity and mortality rates from S. pneumoniae infections are high in the elderly, immunization should be systematically performed. However, the elderly subject is frequently immunosuppressed, and it is therefore advisable to control the result of the vaccination, using a simple procedure, such as the one described here.

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References

  1. AmmannA.J., SchiffmanG. and AustrianR. (1980): The antibody response to pneumococcal capsular polysaccharides in aged individuals. - Proc. Soc. Exp. Biol. Med. 164: 312–316.

    Google Scholar 

  2. ArmstrongJ.R. and NarasimhanS.L. (1979): Drug-resistant pneumococci. - New Eng. J. Med. 300: 499–500.

    Google Scholar 

  3. AustrianR. (1977): Prevention of pneumococcal infection by immunization with capsular polysaccharides of Streptococcus pneumoniae: current status of polyvalent vaccines. - J. Infect. Dis. 136: 538–542.

    Google Scholar 

  4. AustrianR. (1980): The assessment of pneumococcal vaccine. - New. Engl. J. Med. 303: 578–580.

    Google Scholar 

  5. AzoulayG., HenryJ.F., ForettiF., BerthauxP. and AcarJ.F. (1977): Les septicémies du vieillard. - Nouv. Presse Med. 6: 3075–3082.

    Google Scholar 

  6. BairdD.R. and WhittleH.C. (1976): Mortality pneumococcal meningitis. - Lancet 2: 1344–1346.

    Google Scholar 

  7. BorgonoJ.M., McLeanA.A., VellaP.P., WoodhourA.F., CanepaI., DavidsonW.L. and HillemanM.R. (1978): Vaccination and revaccination with polyvalent pneumococcal polysaccharide vaccines in adult and infants. - Proc. Soc. Exp. Biol. Med. 157: 148–154.

    Google Scholar 

  8. BuckleyC.E., BuckleyE.G. and DorseyF.C. (1974): Longitudinal changes in serum immunoglobulin levels in older humans. - Fed. Proc. 33: 2036–2039.

    Google Scholar 

  9. CallahanL.T., WoodhourA.F., MeekerJ.B. and HillemanM.R. (1979): Enzyme-linked immunosorbent assay for measurement of antibodies against pneumococcal polysaccharide antigens: comparison with radioimmunoassay. - J. Clin. Microbiol. 10: 459–463.

    Google Scholar 

  10. GoldsteinF., DangVanA., BouanchaudD. and AcarJ.F. (1988): Evolution de la résistance aux antibiotiques des pneumocoques et répartition de leurs types capsulaires. - Path. Biol. 26: 173–180.

    Google Scholar 

  11. HansmanD. (1975): Antibiotic sensitivity pattern of pneumococci relatively insensitive to penicillin and cephalosporin antibiotics. - Med. J. Aust. 2: 740–742.

    Google Scholar 

  12. O'ShannessyD.J. and QuarlesR.H. (1987): Labeling of the oligosaccharide moieties of immunoglobulins. -J. Immunol. Meth. 99: 153–161.

    Google Scholar 

  13. ParkerM.T. (1978): Multiply resistant Pneumococcus. -Lancet 15: 155–156.

    Google Scholar 

  14. QuaadeF. and ChristensenK. (1962): Purulent meningitis. A review of 658 cases. - Acta Med. Scand. 171: 543–550.

    Google Scholar 

  15. SchiffmanG., DouglasR.M., BonnerM.J., RobbinsM. and AustrianR. (1980): A radioimmunoassay for immunologic phenomena in pneumococcal disease and for the antibody response to pneumococcal vaccines. I. Method for the radioimmunoassay of anticapsular antibodies and comparison with other techniques. - J. Immunol. Meth. 33: 133–144.

    Google Scholar 

  16. SmitP., OberholzerD., Hayden-SmithS., KoornhofH. and HillemanM.R. (1977): Protective efficacy of pneumococcal polysaccharide vaccines. - J. Am. Med. Assoc. 238: 2613–2616.

    Google Scholar 

  17. VerheulA.F.M., VersteegA.A., deReuverJ., JanszeM. and SnippeH. (1989): Modulation of the immune response to pneumococcal type 14 capsular polysaccharide-protein conjugates by the adjuvant Quil A depends on the properties of the conjugates. -Infect. Immun. 57: 1078–1083.

    Google Scholar 

  18. WeibelR.E., VellaP.P., McLeanA.A., WoodhourA.F., DavidsonW.L. and HillemanM.R. (1977): Studies in human subjects of polyvalent pneumococcal vaccines. - Proc. Soc. Exp. Biol. Med. 156: 144–150.

    Google Scholar 

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Authors and Affiliations

  1. Laboratory of Toxin Immunochemistry, Pasteur Institute, 25 et 28, Rue du Docteur Roux, 75724, Paris, Cedex 15

    M. Fattal-German & B. Bizzini

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  1. M. Fattal-German
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  2. B. Bizzini
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Fattal-German, M., Bizzini, B. Monitoring of the response of elderly individuals to pneumococcal vaccination with the aid of a novel ELISA. Eur J Epidemiol 6, 371–375 (1990). https://doi.org/10.1007/BF00151710

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