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Enzyme-linked immunosorbent assay (ELISA) for detection of thermostable protease from Pseudomonas sp. AFT-36 of dairy origin

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Biotechnology Techniques

Summary

A double-antibody sandwich enzyme-linked immunosorbent 'assay was developed using IgG of anti-Pseudomonas sp. AFT-36 protease as coating antibody. The assay could detect 0.19 ng/ml of protease in milk and buffer. It could be completed within 5 h. Cross-reactivity studies showed that AFT-36 is immunologically related to six Pseudomonas species, while some Pseudomonas spp.produce immunologically unrelated proteases. The results suggest that the assay could be used to detect protease in dairy products.

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Authors and Affiliations

  1. Department of Microbiology, College of Basic Sciences, Himachal Pradesh Agricultural University, 176 062, Palampur, H.P., India

    V. Punj & Hittu Matta

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  1. V. Punj
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  2. Hittu Matta
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Punj, V., Matta, H. Enzyme-linked immunosorbent assay (ELISA) for detection of thermostable protease from Pseudomonas sp. AFT-36 of dairy origin. Biotechnol Tech 9, 283–288 (1995). https://doi.org/10.1007/BF00151576

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