Skip to main content
SpringerLink
Log in

Effect of differentiation inducers on growth characteristics of human glioma cell lines

  • Published:
Journal of Neuro-Oncology Aims and scope Submit manuscript

Summary

The effect of dimethylsulfoxide (DMSO) and iododeoxyuridine (IUdR) on the growth characteristics of two established human glioblastoma cell lines (FG and HMCN-1) was studied. The FG cell line has been characterized. The HMCN-1 cell line, established in our laboratory, consisted of fibroblastoid and polygonal cells that grew without contact inhibition. Subcutaneous injection of these cells into weanling athymic nude mice induced slowly growing, solid tumors that were histologically spindly with areas that were similar to the original tumor. Chromosomal analyses revealed a human heteroploid pattern with a modal number of 69. The cells of the original human glioma contained S-100 protein and glial fibrillary acidic protein (GFA protein), whereas the established cells failed to express markers. Prolonged treatment of glioma cells with DMSO generated a more adherent, normal human fibroblastoid phenotype that grew with contact inhibition. The new phenotype and proliferative restriction of these cells was evident as late as 50 days after discontinuation of treatment. The chemical induction of cell differentiation resulted in decreased tumorigenic potential in athymic nude mice.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Friend C, Scher W, Holland JG, Sato T: Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethylsulfoxide. Proc Natl Acad Sci USA 68:378–382, 1971

    Google Scholar 

  2. Boyer SH, Wuu KD, Noyes AN, Young R, Scher W, Friend C, Preisler HD, Bank A: Hemoglobin biosynthesis in murine virus-induced leukemic cells in vitro: structure and amounts of globin chains produced. Blood 40:823–835, 1972

    Google Scholar 

  3. Ross J, Ikawa Y, Leder P: Globin messenger-RNA induction during erythroid differentiation of cultured leukemia cells. Proc Natl Acad Sci USA 69:3620–3623, 1972

    Google Scholar 

  4. Tereda M, Fried J, Nudel U, Rifkind RA, Marks PA: Transient inhibition of initiation of S-phage associated with dimethylsulfoxide induction of murine erythroleukemia cells to erythroid differentiation. Proc Natl Acad Sci USA 74:248–252, 1977

    Google Scholar 

  5. Collins SJ, Ruscetti FW, Gallagher RE, Gallo RC: Terminal differentiation of human promyelocytic leukemia cells induced by dimethylsulfoxide and other polar compounds. Proc Natl Acad Sci USA 75:2458–2462, 1978

    Google Scholar 

  6. McKeever PE, Quindlen E, Banks MA, Williams U, Kornblith PL, Laverson S, Greenwood MA, Smith B: Biosynthesized products of cultured neuroglial cells. I. Selective release of proteins by cells from human astrocytomas. Neurology 31:1445–1152, 1981

    Google Scholar 

  7. Shitara N, McKeever PE, Whang-Peng J, Knutsen T, Smith BH, Kornblith PL: Flow cytometric and cytogenic analysis of human cultured cell lines derived from high-low grade astrocytoma. Acta Neuro Pathol (Berlin) 60:40–48, 1983

    Google Scholar 

  8. Caspersson T, Lomakka G, Zech L: The 24 fluorescence patterns of the human metaphase chromosomes: distinguishing characters and variability. Hereditas 67:89–94, 1971

    Google Scholar 

  9. Borenfreund E, Steinglass M, Korngold G, Bendich A: Effect of dimethylsulfoxide and dimethylformamide on the growth and morphology of tumor cells. Ann NY Acad Sci 243:164–171, 1975

    Google Scholar 

  10. Kimhi Y, Palfrey C, Spector I, Barak Y, Littauer UZ: Maturation of neuroblastoma cells in the presence of dimethylsulfoxide. Proc Natl Acad Sci USA 73:462–466, 1976

    Google Scholar 

  11. Kim YS, Tsao D, Siddiqui B, Whitehead JS, Arnstein P, Bennett J, Hicks J: Effects of sodium butyrate and dimethylsulfoxide on biochemical properties of human colon cancer cells. Cancer 45:1185–1192, 1980

    Google Scholar 

  12. Tralka TS, Rabson AS: Cilia formation in cultures of human lung cancer cells treated with dimethylsulfoxide. J Natl Cancer Inst, 57:1383–1388, 1976

    Google Scholar 

  13. Fulling KH, Nelson JS: Cerebral astrocytic neoplasms in the adult: contribution of histologic examination to the assessment of prognosis. Sem Diagn Pathol 1:152–163, 1984

    Google Scholar 

  14. Frankel S, German WJ: Glioblastoma multiforme: review of 219 cases with regard to natural history, pathology, diagnostic methods and treatment. J Neurosurg 15:489–503, 1958

    Google Scholar 

  15. Scherer HJ: The forms of growth in gliomas and their practical significance. Brain 63:1–35, 1940

    Google Scholar 

  16. Bigner DD, Bigner SH, Poten J, Westermark B, Mahaley MS, Ruoslathi E, Herschman H, Eng LF, Wikstrand CJ: Heterogeneity of genotypic and phenotype characteristics of 15 permanent cell lines derived from human gliomas. J Neuropath Exp Neurol 40:201–229, 1981

    Google Scholar 

  17. Bigner SH, Bullard DE, Pegram CN, Wikstrand CJ, Bigner DD: Relationship of in vitro morphologic and growth characteristics of established human glioma-derived cell lines to their tumorgenicity in athymic nude mice. J Neuropath Exp Neurol 40:390–409, 1981

    Google Scholar 

  18. Jones TR, Bigner SH, Schold SC, Eng LF, Bigner DD: Anaplastic human gliomas grown in athymic mice. Am J Pathol 105:316–327, 1981

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Neurology, The Milton S. Hershey Medical Center, The Pennsylvania State University, 17033, Hershey, Pennsylvania, USA

    Laszlo Geder & Robert W. Brennan

  2. Department of Pathology, The Milton S. Hershey Medical Center, The Pennsylvania State University, 17033, Hershey, Pennsylvania, USA

    John W. Kreider

  3. Division of Neuropathology, The Milton S. Hershey Medical Center, The Pennsylvania State University, 17033, Hershey, Pennsylvania, USA

    Javad Towfighi

  4. Division of Neurosurgery, The Milton S. Hershey Medical Center, The Pennsylvania State University, 17033, Hershey, Pennsylvania, USA

    Robert B. Page

  5. Department of Pediatrics, The Milton S. Hershey Medical Center, The Pennsylvania State University, 17033, Hershey, Pennsylvania, USA

    Roger L. Ladda

  6. Department of Microbiology, The Milton S. Hershey Medical Center, The Pennsylvania State University, 17033, Hershey, Pennsylvania, USA

    Fred Rapp

Authors
  1. Laszlo Geder
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. John W. Kreider
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Javad Towfighi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Robert B. Page
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Roger L. Ladda
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Robert W. Brennan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Fred Rapp
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Geder, L., Kreider, J.W., Towfighi, J. et al. Effect of differentiation inducers on growth characteristics of human glioma cell lines. J Neuro-Oncol 5, 251–271 (1987). https://doi.org/10.1007/BF00151230

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00151230

Key words

Search

Navigation