Abstract
A pot experiment was conducted in a 14C-labelled atmosphere to study the influence of living plants on organic-N mineralization. The soil organic matter had been labelled, by means of a 200-days incubation, with 15N. The influence of the carbon input from the roots on the formation of microbial biomass was evaluated by using two different light intensities (I). Mineralization of 15N-labelled soil N was examined by following its fate in both the soil biomass and the plants. Less dry matter accumulated in shoots and roots at the lower light intensity. Furthermore, in all the plant-soil compartments examined, with the exception of rhizosphere respiration, the proportion of net assimilated 14C was lower in the low-I treatment than in the high-I treatment. The lower rates of 14C and 15N incorporation into the soil biomass were associated with less root-derived 14C. During the chamber period (14CO2-atmosphere), mineralized amounts of 15N (measured as plant uptake of 15N) were small and represented about 6.8 to 7.8% of the initial amount of organic 15N in the soil. Amounts of unlabelled N found in the plants, as a percentage of total soil N, were 2.5 to 3.3%. The low availability of labelled N to microorganisms was the result of its stabilization during the 210 days of soil incubation. Differences in carbon supply resulted in different rates of N mineralization which is consistent with the hypothesis that roots induce N mineralization. N mineralization was higher in the high-I treatment. On the other hand, the rate of mineralization of unlabelled stable soil N was lower than labelled soil 15N which was stabilized. The amounts of 15N mineralized in planted soil during the chamber period (43 days) which were comparable with those mineralized in unplanted soil incubated for 210 days, also suggested that living plants increased the turnover rate of soil organic matter.
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Billes, G, Bottner, P and Gandais-Riollet, N 1988 Effet des racines de graminées sur la minéralisation nette de l'azote du sol. Rev. Écol. Biol. Sol 25, 261–277.
Breland, T A and Bakken, L R 1991 Microbial growth and nitrogen immobilization in the root zone of barley (Hordeum vulgare L.), Italian ryegrass (Lolium mutiflorum Lam.) and white clover (Trifolium repens L.). Biol Fertil. Soils 12, 154–160.
Bremner, J M 1965 Total nitrogen. In Methods of Soil Analysis, Part 2. Eds. C ABlack et al. Agronomy 9, pp 1149–1178. Am. Soc. Agron. Inc. Madison, Wis.
Bremner, J M and Mulvaney, C S 1982 Nitrogen-total. In Methods of Soil Analysis, Part 2. Eds. A LPage, R HMiller and D RKeeney. Agronomy 9, pp 595–624. Am. Soc. Agron. Inc. Madison. Wis.
Brookes, P C, Landman, A, Pruden, G and Jenkinson, D S 1985 Chloroform fumigation and the release of soil nitrogen: A rapid direct extraction method to measure microbial biomass nitrogen in soil. Soil Biol. Biochem. 17, 837–842.
Chaussod, R, Houot, S, Guiraud, G and Hetier, J M 1988 Size and turnover of the microbial biomass in agricultural soils: Laboratory and field experiments. In Nitrogen Efficiency in Agricultural Soils. Eds. D SJenkinson and K ASmith. pp 312–326. Elsevier, England.
Clarholm, M 1985 Possible roles for roots, bacteria, protozoa and fungi in supplying nitrogen to plants. In Ecological Interactions in the Soil. Eds. A HFitter, DAtkinson, D JRead and M BUsher. Special Publication No 4 of the British Ecological Society, pp 355–365. Blackwell, Oxford.
Clarholm, M 1989 Effects of plant-bacterial-amoebal interactions on plant uptake of nitrogen under field conditions. Biol. Fertil. Soils 8, 373–378.
Corré, W J 1983 Growth and morphogenesis of sun and shade plants I. The influence of light intensity. Acta Bot. Neerl. 32, 49–62.
Elliot, E T, Coleman, D C, Ingham, R E and Trofymow, J A 1984 Carbon and energy flow through microflora and microfauna in the soil subsystem of terrestrial ecosystems. In Current Perspectives in Microbial Ecology. Eds. M JKlug and C AReddy. pp 424–433. American Society for Microbiology, Washington, D.C.
Griffiths, B and Robinson, D 1992 Root-induced nitrogen mineralisation: A nitrogen balance model. Plant and Soil 139, 253–263.
Haider, K, Mosier, A and Heinemeyer, O 1987 The effect of growing plants on denitrification at high soil nitrate concentrations. Soil Sci. Soc. Am. J. 51, 97–102.
Haider, K, Mosier, A and Heinemeyer, O 1991 Effects of plants on nitrogen and carbon turnover in soil. In Diversity of Environmental Biogeochemistry. Ed. JBerthelin. Developments in Geochemistry 6. pp 419–426. Elsevier, Amsterdam.
Helal, H M and Sauerbeck, D 1984 Influence of plant roots on C and P metabolism in soil. Plant and Soil 76, 175–182.
Helal, H M and Sauerbeck, D 1986 Effect of plant roots on carbon metabolism of soil microbial biomass. Z. Pflanzenernaehr. Bodenkd. 149, 181–188.
Helal, H M and Sauerbeck, D 1987 Direct and indirect influences of plant roots on organic matter and phosphorus turnover in soil. INTECOL Bulletin 15, 49–58.
Helal, H M and Sauerbeck, D 1989 Carbon turnover in the rhizosphere. Z. Pflanzenernaehr. Bodenkd. 152, 211–216.
Ivarsson, K and Bjarnason, S 1988 The long-term soil fertility experiments in southern Sweden. Acta Agric. Scand. 38, 137–143.
Jansson, S L and Persson, J 1982 Mineralization and immobilization of soil nitrogen. In Nitrogen in Agricultural Soils. Ed. F JStevenson. Agronomy 22, pp 229–252. Am. Soc. Agron. Inc. Madison, Wis.
Jenkinson, D S 1988 Determination of microbial biomass carbon and nitrogen in soil. In Advances in Nitrogen Cycling in Agricultural Ecosystems. Ed. J RWilson. pp 368–386. C. A. B. International, Wallingford.
Jenkinson, D S, Fox, R H and Rayner, J H 1985 Interactions between fertilizer nitrogen and soil nitrogen-the so-called ‘priming effect’. J. Soil Science 36, 425–444.
Johansson, G 1991 Carbon distribution in meadow fescue (Festuca pratensis L.) determined in a growth chamber with 14C-labelled atmosphere. Acta Agric. Scand. 41, 37–46.
Keeney, D R and Nelson, D W 1982 Nitrogen-inorganic forms. In Methods of Soil Analysis, Part 2. Eds. A LPage, R HMiller and D RKeeney. Agronomy, 9, pp 643–698. Am. Soc. Agron. Inc. Madison, Wis.
Kirchmann H and Eriksson J 1993 Properties and classification of soils of the Swedish long-term fertility experiments: II. Sites at Örja and Orup. Acta Agric. Scand., Sect. B. Soil and Plant Sci. (In press).
Kuikman, P J and VanVeen, J A 1989 The impact of protozoa on the availability of bacterial nitrogen to plants. Biol. Fertil. Soils 8, 13–18.
Kuiper, D and Smid, A 1985 Genetic differentiation and phenotypic plasticity in Plantago major ssp major: I. The effect of differences in level of irradiance on growth, photosynthesis, respiration and chlorophyll content. Physiol. Plant. 65, 520–528.
Lambers, H and Posthumus, F 1980 The effect of light intensity and relative humidity on growth rate and root respiration of Plantago lanceolata and Zea mays. L. Exp. Bot. 31, 1621–1630.
Liljeroth, E, VanVeen, J A and Miller, H J 1990 Assimilate translocation to the rhizosphere of two wheat lines and subsequent utilization by rhizosphere microorganisms at two soil nitrogen concentrations. Soil Biol. Biochem. 22, 1015–1021.
Martin, J K 1987 Effects of plants on the decomposition of 14C-labelled soil organic matter. Soil Biol. Biochem. 19, 473–474.
Merckx, R, Dijkstra, A, DenHartog, A and VanVeen, J A 1987 Production of root-derived material and associated microbial growth in soil at different nutrient levels. Biol. Fertil. Soils 5, 126–132.
Reid, J B and Goss, M J 1983 Growing crops and transformations of 14C-labelled soil organic matter. Soil Biol. Biochem. 6, 687–691.
Sallih, Z, Bottner, P, Billès, G and Soto, P 1987 Interation racines-microorganismes: carbone et azote de la biomasse microbienne dévelopée en présence de racines. Rev. Écol. Biol. Sol 24, 459–471.
SAS Institute INC: SAS/STAT User's Guide, version 6, Fourth Edition, Vol. 2. Cary, NC: SAS Institute Inc., 1989, 846 p.
Sattelmacher, B, Gerendas, J, Thoms, K, Brück, H and Bagdady, N H 1993 Interaction between root growth and mineral nutrition. Environ. Exp. Bot. 33, 63–73.
Sparling, G P, Cheshire, M V and Mundie, C M 1982 Effect of barley plants on the decomposition of 14C-labelled soil organic matter. J. Soil Sci. 33, 89–100.
Ta, T C and Ohira, K 1981 Effects of various environmental and medium conditions on the response of Indica and Japonica rice plants to ammonium and nitrate nitrogen. Soil Sci. Plant Nutr. 27, 347–355.
Vance, E D, Brookes, P C and Jenkinson, D S 1987 An extraction method for measuring soil microbial C. Soil Biol. Biochem. 19, 703–707.
Wheatley, R, Ritz, K and Griffiths, B 1990 Microbial biomass and mineral N transformations in soil planted with barley, ryegrass, pea and turnip. Plant and Soil 127, 157–167.
Zadoks, J C, Chang, T T and Konzak, C F 1974 A decimal code for the growth stages of cereals. Weed Research 14, 415–421.
Zagal, E 1993 Measurement of microbial biomass in rewetted air-dried soil by fumigation-incubation and fumigation-extraction techniques. Soil Biol. Biochem. 25, 553–559.
Zagal, E, Bjarnasson, S and Olsson, U 1993 Carbon and nitrogen in the root-zone of barley (Hordeum vulgare L.). Plant and Soil 157, 51–63.
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Zagal, E. Influence of light intensity on the distribution of carbon and consequent effects on mineralization of soil nitrogen in a barley (Hordeum vulgare L.)-soil system. Plant Soil 160, 21–31 (1994). https://doi.org/10.1007/BF00150342
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DOI: https://doi.org/10.1007/BF00150342