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Genetic construction of Xanthomonas campestris and xanthan gum production from whey

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Summary

Plasmids pUR291 and pNZ521 containing lacZ gene, maturation protein and proteinase P genes, were transferred into X. campestris either by conjugation or by transformation. Plasmid pNZ521 was also conjugally transferred into X. campestris XMT1 a transformant carrying plasmid pUR291. All the constructed strains were evaluated for xanthan gum production in either a medium of 50% whey or the same medium supplemented with 1.5% lactose or 1.5% glucose. Mixed cultures either with transconjugants or with transformants were tested for xanthan gum production as well.

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Papoutsopoulou, S.V., Ekateriniadou, L.V. & Kyriakidis, D.A. Genetic construction of Xanthomonas campestris and xanthan gum production from whey. Biotechnol Lett 16, 1235–1240 (1994). https://doi.org/10.1007/BF00149624

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  • DOI: https://doi.org/10.1007/BF00149624

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