Summary
An examination was made of the effects of four different starvation conditions on a member of the industrially important bacterial genus Streptomyces (Streptomyces lividans). Protein synthesis after the switch from an initial rich medium (YEME) to the different starvation media was examined by [35S]methionine labelling and followed over a time course of 2 hours. Changes in the pattern of protein synthesis were observed which varied according to the different nutrient limitations examined and appeared to suggest the presence of stress protein subsets which were specific to the nutrient limited. The major HSPs previously identified in S.lividans were not observed to be induced by these nutrient limitations.
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References
Lindquist, S. (1986) Annual Reviews in Biochemistry 55, 1151–1191.
Pelham, H.R.B. (1985) Trends in Genetics 1, 31–35.
Neidhardt, F.C. and VanBogelen, R.A. (1987) In: Escherichia coli and Salmonella typhimurium; cellular and molecular biology. Eds. Neidhardt, F.C., Ingraham, J.I., Low, K., Magasanik, B., Schaechter, M. and Umbarger, H.E. American Society for Microbiology, p1334–1345.
Hightower, L.E. (1980) J. Cellular Physiology 102, 407–427.
Ashburner, M. and Bonner, J.J. (1979) Cell 17, 241–254.
Levinson, N., Oppermann, H. and Jackson, J. (1980) Biochim. Biophys. Acta 606, 170–180.
Harder, W. and Dijkhuizen, L. (1983) Annual Reviews in Microbiology 37, 1–23.
Hopwood, D.A., Bibb, M.J., Chater, K.F., Kieser, T., Bruton, C.J., Kieser, H.M., Lydiate, D.J., Smith, C.P., Ward, J.M. and Schrempff, H. (1985) Genetic Manipulation of Streptomyces: a laboratory manual. John Innes Institute, Norwich.
Lomovskaya, N.D., Mkrtumian, N.M., Goshmskaya, N.L. and Danilenko, V.N. (1972) Journal of Virology 9, 258–262.
Rebello, A., Gil, J.A., Liras, P., Asturias, J.A. and Martin, J.A. (1989) Gene 79, 47–58.
Hobbs, G., Frazer, C.M., Gardner, D.C.J., Cullum, J.A. and Oliver, S.G. (1989) Applied Microbial Biotechnology 31, 272–277.
Groat, R.G., Schultz, J.E., Zychlinsky, E., Bockman, A. and Martin, A. (1986) J. Bacteriology 168, 486–493.
Barratt, E.M., Butler, P.R., Hobbs, G. and Oliver, S.G. (1993) (submitted for publication).
Holt, T.G., Chang, C., Laurent-Winter, C., Murakami, T., Garrels, J.I., Davies, J.E. and Thompson, C.J. (1992) Molecular Microbiology 6, 969–980.
Jenkins, D.E., Auger, E.A. and Matin, A. (1991) J. Bacteriology 173, 1992–1996.
Guglielmi, G., Mazodier, P., Thompson, C.J. and Davies, J. (1991) J. Bacteriology 173, 7374–7381.
Matin, A. (1989) Molecular Microbiology 5, 19–22.
Spector, M.P. and Cubitt, C.L. (1991) Molecular Microbiology 6, 1467–1476.
Cashell, M. and Rudd, K.E. (1987) Molecular and Cellular Biology 2, 1410–1438.
Laemmli, U.K. (1970) Nature 227, 680–685.
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Barratt, E.M., Oliver, S.G. The effects of nutrient limitation on the synthesis of stress proteins in Streptomyces lividans . Biotechnol Lett 16, 1231–1234 (1994). https://doi.org/10.1007/BF00149623
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DOI: https://doi.org/10.1007/BF00149623