Abstract
The presence of a highly purified recombinant interleukin-2 (rIL-2) increased the production of immunoglobulin (IgM or IgG) by human-human hybridomas to 1.5–2.0 times the production by untreated cells. However, these cells did not react with anti-Tac (IL-2 receptor α) antibody. To enhance the response of the hybridoma cells to rIL-2, Tac gene was introduced by co-transfection with Tac gene expression plasmid pTB459 and G418 resistant gene expression plasmid pRSVneo. Tac cDNA transfected hybridoma (HBW-4.16.459-6-126) was induced to produce 6 times as much IgG by rIL-2 as was the control. This antibody production promoting phenomenon mediated by rIL-2 was depressed by anti-Tac antibody.
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Iwamoto, K., Shintani, Y., Sasada, R. et al. Improvement in the antibody productivity of human-human hybridomas by transfection with Tac gene. Cytotechnology 4, 29–37 (1990). https://doi.org/10.1007/BF00148808
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DOI: https://doi.org/10.1007/BF00148808