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Thermal stability characteristics of cellulase enzymes produced by Sporotrichum thermophile

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Summary

The thermal stability characteristics of the cellulase enzymes present in culture filtrates of the thermophilic fungus Sporotrichum thermophile were investigated at different temperatures and at different times of exposure. Maximum enzymic activities under assay conditions were found at 68°C for the filter paper activity (FPA) and the Cx activity (carboxymethylcellulose), while the maxima for the C1 activity (cotton) and β-glucosidase activity (cellobiose) were found to be at 55°C and 72°C respectively. Culture filtrates were exposed to a given constant temperature for varying lengths of time to a maximum of 48 hrs. and then analyzed for residual enzymic activities under assay conditions. The exposure temperatures studied were 50°C, 60°C and 65°C. After 48 hrs. exposure time at 50°C the residual activities for the FPA, Cx and β-glucosidase were found to be 88%, 98% and 93% of the original activities respectively.

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Authors and Affiliations

  1. Chemical and Biochemical Engineering Faculty of Engineering Science, The University of Western Ontario, N6A 5B9, London, Ontario, Canada

    Argyrios Margaritis & Edward Creese

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  1. Argyrios Margaritis
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  2. Edward Creese
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Margaritis, A., Creese, E. Thermal stability characteristics of cellulase enzymes produced by Sporotrichum thermophile . Biotechnol Lett 3, 471–476 (1981). https://doi.org/10.1007/BF00147556

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  • DOI: https://doi.org/10.1007/BF00147556

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