Skip to main content
Log in

Immobilization of hydrogenases for biophotolytic hydrogen production; stability and kinetics

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

Hydrogenases from Clostridium pasteurianum and Desulfovibrio desulfuricans were immobilized on solid supports with retention of 50% activity. The immobilized enzymes were more stable than the free enzymes and were active in the biophotolytic hydrogen production from water.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Fitzgerald, M.P., Rogers, L.J., Rao, K.K. and Hall, D.O. (1980) Biochem. J. 192, 665–672.

    Google Scholar 

  • Gisby, P.E. and Hall, D.O. (1980) Nature 287, 251–253.

    Google Scholar 

  • Hall, D.O. and Rao, K.K. (1977) Ferredoxin. In: Encyclopedia of Plant Physiology, A. Trebst and M. Avron eds. Vol.5, pp 206–216. Berlin: Springer-Verlag.

    Google Scholar 

  • Hesse, G. (1973) Acta Histochem. 46, 253–266.

    Google Scholar 

  • Lappi, D.A., Stolzenbach, F.A., Kaplan, N.O. and Kamen, M.D. (1976) Biochem. Biophys. Res. Comm. 69, 878–884.

    Google Scholar 

  • Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951) J. Biol. Chem. 193, 265–275.

    Google Scholar 

  • Monsan, P. (1978) J. Mole. Catalysis 3, 371–384.

    Google Scholar 

  • Mossbach, K. ed. (1976) Immobilized Enzymes. Methods in Enzymology. 44.

  • Rao, K.K., Gogotov, I.N. and Hall, D.O. (1978) Biochimie 60, 291–296.

    Google Scholar 

  • Rao, K.K., Rosa, L. and Hall, D.O. (1976) Biochem. Biophys. Res. Comm. 68, 21–27.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Plasterk, R.H.A., Rao, K.K. & Hall, D.O. Immobilization of hydrogenases for biophotolytic hydrogen production; stability and kinetics. Biotechnol Lett 3, 99–104 (1981). https://doi.org/10.1007/BF00145118

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00145118

Keywords

Navigation