Summary
Hydrogenases from Clostridium pasteurianum and Desulfovibrio desulfuricans were immobilized on solid supports with retention of 50% activity. The immobilized enzymes were more stable than the free enzymes and were active in the biophotolytic hydrogen production from water.
Similar content being viewed by others
References
Fitzgerald, M.P., Rogers, L.J., Rao, K.K. and Hall, D.O. (1980) Biochem. J. 192, 665–672.
Gisby, P.E. and Hall, D.O. (1980) Nature 287, 251–253.
Hall, D.O. and Rao, K.K. (1977) Ferredoxin. In: Encyclopedia of Plant Physiology, A. Trebst and M. Avron eds. Vol.5, pp 206–216. Berlin: Springer-Verlag.
Hesse, G. (1973) Acta Histochem. 46, 253–266.
Lappi, D.A., Stolzenbach, F.A., Kaplan, N.O. and Kamen, M.D. (1976) Biochem. Biophys. Res. Comm. 69, 878–884.
Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951) J. Biol. Chem. 193, 265–275.
Monsan, P. (1978) J. Mole. Catalysis 3, 371–384.
Mossbach, K. ed. (1976) Immobilized Enzymes. Methods in Enzymology. 44.
Rao, K.K., Gogotov, I.N. and Hall, D.O. (1978) Biochimie 60, 291–296.
Rao, K.K., Rosa, L. and Hall, D.O. (1976) Biochem. Biophys. Res. Comm. 68, 21–27.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Plasterk, R.H.A., Rao, K.K. & Hall, D.O. Immobilization of hydrogenases for biophotolytic hydrogen production; stability and kinetics. Biotechnol Lett 3, 99–104 (1981). https://doi.org/10.1007/BF00145118
Issue Date:
DOI: https://doi.org/10.1007/BF00145118