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Chlamydia specific IgG and IgA antibodies in women with obstructive infertility as determined by immunoblotting and assays

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Abstract

The prevalence rate of IgG and IgA antibodies to Chlamydia was analyzed in 50 women with laparoscopy-verified tubal infertility and in 50 age-matched control women by single serovar (L2) inclusion immunoperoxidase assay (IPA) and by immunoblotting technique (IB) Women with tubal infertility had significantly (p < 0.001) elevated IPA Chlamydia IgG antibody titer ≥ 128 and ≥ 256 than controls (64% vs 16%. Odds ratio = 9.3 and 50% vs 10%, Odds ratio = 9 respectively). The prevalence rate of IPA IgA antibody titer (≥ 16) to Chlamydia was also significantly higher (p < 0.001) in women with tubal infertility than controls (48% vs 8%, Odds ratio = 10:6).

Antibodies to at least 19 chlamydial structural polypeptides ranging in molecular weight from 30 kD to 204 kD, were detected by the IB technique in the IPA seropositive sera. Antibodies to 57-60 kD were detectable in almost all the IPA IgG and IgA seropositive sera. The prevalence rate of IgG antibody to 57 kD-60 kD was significantly higher in women with obstructive infertility than healthy woman (84% vs. 56% p < 0.01; Odds ratio = 3.8). More significantly, higher differences to 57–60 kD polypeptide were found in the case of IgA between the infertile women and controls (52% vs. 10%, p < 0.001;; Odds ratio = 9.7). The significance of IPA and IB technique for screening of infertile women is discussed.

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References

  1. BrunhamR., MacleanI., BinnsB., PeelingR. (1985): Chlamydia trachomatis: Its Role in Tubal Infertility. - J. Infect. Dis. 152: 1275–1282.

    Google Scholar 

  2. CaldwellH.D., KuoC.C., KennyG.E. (1975): Antigenic analysis of chlamydiae by two-dimensional immunoelectrophoresis. II. A trachoma-LGV-specific antigen. - J. Immunol. 115: 969–975.

    Google Scholar 

  3. CeveniniR., PossatiG., LaPlacaM. (1982): Chlaricydia trachomatis infection in infertile women, in Mardh P.A., Holmes K.K., Oriel J.K., Piot P. and Schachter J. (eds): Chlamydial Infections. Elsevier Biomedical Press, Amsterdam. pp. 189–192.

    Google Scholar 

  4. CeveniniR., SarovI., RumpianesiF., DonatiM., MelegaC., VarrottiC. and LaPlacaM. (1984): Serum specific IgA antibody to Chlamydia trachomatis in patients with chlamydial infections, detected by ELISA and immunofluorescence test. -J. Clin. Pathol. 37: 686–691.

    Google Scholar 

  5. ConwayD., GlazenerC.M.A., CaulE.O., HodgsonJ., HullM.G.R., ClarkS.K.R., StirratG.M. (1984): Chlamydial serology in fertile and infertile women.- Lancet 1: 191–193.

    Google Scholar 

  6. CsangoP.A., SarovB., SchiotzH. and SarovI. (1988): Chlamydia trachomatis isolation and the comparative diagnostic value of serology in women seeking abortion. - J. Clin. Pathol. 91: 89–92.

    Google Scholar 

  7. ForseyT., StainsbyK., HogerP.H., RidgewayG.L., DarougarS., Fischer-BruggeU. (1986): Comparison of two immunofluorescence tests for detecting antibodies to C. trachomatis. - Eur. J. Epidemiol. 2: 163–164.

    Google Scholar 

  8. Henry-SuchetJ., CatalanF., LoffredoV., SansonM.J., DebacheC., PigeauF., CoppinR. (1981): Chlamydia trachomatis associated with chronic inflammation in abdominal specimens from women selected for tuboplastry. - Fertil. Steril. 36: 599–605.

    Google Scholar 

  9. Henry-SuchetG., CatalanF., ParisX. and LoffredoV. (1982): Antibody titer to Chlamydia trachomatis in acute salpingitis and obstructive sterilities. In: Mardh P.A., Holmes K.K., Oriel J.K., Piot P. and Schachter J. (eds) Chlamydial Inflections. pp. 183–187. Elsevier Biomedical Press, Amsterdam.

    Google Scholar 

  10. JonesB.R., ArderyB.R., HuiS.L. and ClearyR.E. (1982): Correlation between serum antichlamydial antibodies and tubal factor as a cause of infertility.- Fert. Steril., 38: 553–558.

    Google Scholar 

  11. Kaneti J., Sarov B. and Sarov I. ( ): IgG and IgA antibodies specific for Chlamydia trachomatis in acute epididymitis. - Eur. Urol. In Press.

  12. KaulR., WenemanW.M. (1985): Cloning and expression in Escherichia coli of a species-specific Chlamydia trachomatis outer membrane antigen. -FEMS Microbiology Letters, 27: 7–12.

    Google Scholar 

  13. 13.LadanyS. and SarovI. (1985): Recent advances in Chlamydia trachomatis. - Eur J. Epidemiol., 1: 235–236.

    Google Scholar 

  14. LaemmliU.K. (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T-4. - Nature (London) 227: 680–685.

    Google Scholar 

  15. MacDonaldA.B. (1985): Antigens of Chlamydia trachomatis. - Rev. Infect. Dis. 7: 731–736.

    Google Scholar 

  16. MooreD.E., FoyH.M., DalingJ.R., GraystonJ.T., SpandonyL.R., Wang-S-P., KuaC-C and EschenbachD.A. (1982): Chlamydia trachomatis in infertility due to distal tubal diseases. - Lancet, 2: 574–577.

    Google Scholar 

  17. NewhallV.W.J., BatteigerB., JonesR.B. (1982): Analysis of the human serological response to proteins of Chlamydia trachomatis. - Inflect. Immun. 38: 1181–1189.

    Google Scholar 

  18. PiuraB., SarovL, SarovB., KleinmanD., ChaimW. and InslerV. (1985): Serum IgG and IgA antibodies specific for Chlamydia trachomatis in salpingitis patients as determined by the immunoperoxidase assay. - Europ. J. Epidem. 1: 110–111.

    Google Scholar 

  19. PunnonenR., TerhoP., NikkanenV., MeurmanD. (1979): Chlamydial serology in infertile women by immunofluorescence. - Fertil. Steril. 31: 659–659.

    Google Scholar 

  20. RipaK.T., MardhP-A. (1970): Cultivation of Chlamydia trachomatis in cycloheximide-treated McCoy cells. - J. Clin. Microbiol., 6: 328–331.

    Google Scholar 

  21. SalariS.H. and WardM.E. (1981): Polypeptide composition of Chlamydia trachomatis. - J. Gen. Microbiol., 123: 197–207.

    Google Scholar 

  22. SarovI. and BackerY. (1968): RNA in the elementary bodies of trachoma agent. - Nature, 217: 849–852.

    Google Scholar 

  23. SarovI. and BeckerY. (1969): Trachoma agent DNA. - J. Mot. Biol., 49: 581–589.

    Google Scholar 

  24. SarovL, FriedmanM., LeviE., PascalL., AymardM., BosshardS., ChardonnetY. and RevillardJ.P. (1984): Detection of recurrent cytomegalo virus infection in renal-transplant patients. - J. Infect. Dis. 149: 277.

    Google Scholar 

  25. SarovL, KleinmanD., CeveniniR., HolcbergG., PotashnikG., InslerV. and SarovB. (1986): Specific IgG and IgA antibodies to Chlamydia trachomatis in infertile women. - Int. J. Fert. 31: 193–197.

    Google Scholar 

  26. SarovI., InslerV., SarovB., VeveniniR., RumpianesiR., DonatiM., KleinmanD., PiuraB., LiebermanJ., KimmelN., FriedmanM. and La PlacaM. (1984): Specific serum IgA antibodies in the diagnosis of active viral and chlamydial infections. In: New Horizons in Microbiology. Elsevier Biomedical Press, Amsterdam. Eds. Sanna A., Morace G. pp. 157–168.

    Google Scholar 

  27. Sarov L, Sarov B., Hanuka N., Glasner M. and Kaneti J. (1986): The significance of serum specific IgA antibodies in diagnosis of active Chlamydia trachomatis infections. In: Chlamydial infections. Cambridge University Press. eds. Oriel D., Ridgeway G., Schachter J. et al., pp. 566–569.

  28. SweetR.L., DraperD.L., SchachterJ. (1980): Microbiology and pathogenesis of acute salpingitis as determined by laparoscopy: what is the appropriate site to sample. - Am. J. Obstet. Gynocol. 138: 985.

    Google Scholar 

  29. SweetR.L. (1982): Chlamydial salpingitis and infertility. - Fertil. Steril. 38: 530–533.

    Google Scholar 

  30. SweetR.L., SchachterJ. and LandersD.V. (1983): Chlamydial infections in obstetrics and gynecology.- Clin. Obstet. Gynecol., 26: 143–164.

    Google Scholar 

  31. TowbinH.T., StaehellinT., GordonJ. (1979): Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. - Proc. Natl. Acad. Sci. USA: 76: 4350–4354.

    Google Scholar 

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Authors and Affiliations

  1. Virology, Faculty of Health Sciences, Ben Gurion University of the Negev, 84 105, Beer Sheva

    I. Sarov, N. Hanuka & R. Rosenzeig

  2. Epidemiology Units, Faculty of Health Sciences, Ben Gurion University of the Negev, 84 105, Beer Sheva

    B. Sarov

  3. Division of Obstetrics and Gynecology, Soroka Medical Center, Beer Sheva, Israel

    E. Lunenfeld, G. Potashnik, W. Chaim & V. Insler

Authors
  1. I. Sarov
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  2. E. Lunenfeld
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  3. B. Sarov
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  4. N. Hanuka
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  5. R. Rosenzeig
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  6. G. Potashnik
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  7. W. Chaim
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  8. V. Insler
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Sarov, I., Lunenfeld, E., Sarov, B. et al. Chlamydia specific IgG and IgA antibodies in women with obstructive infertility as determined by immunoblotting and assays. Eur J Epidemiol 4, 216–223 (1988). https://doi.org/10.1007/BF00144755

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