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Plasmid typing of Shigella sonnei epidemic strains and molecular relationship of their R-plasmids

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Abstract

We conducted a surveillance program on epidemic and/or endemic Shigella strains in Asturias (Spain), their frequency and dispersion in our community, and their R-plasmids. We analyzed initial isolates of Shigella sonnei from two epidemic outbreaks using antibiotic resistance patterns and plasmid profile analysis as epidemiological markers. We found that the 2 outbreaks were caused by different S. sonnei strains, which respectively carried one and two R-plasmids together with other plasmids. The molecular relationship among these and three other R-plasmids from two S. sonnei strains isolated during a previous outbreak, were studied by restriction enzyme analysis and DNA-DNA hybridizations. We were able to establish different levels of relationship among the six R-plasmids.

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Authors and Affiliations

  1. Departamento de Biologia Funcional. Area Microbiologia, Universidad de Oviedo, Julian Claveria s/n, 33006, Oviedo, Spain

    M. C. Mendoza, A. J. Gonzalez & F. J. Mendez

  2. Servicio de Microbiología, Hospital Nuestra Señora de Covadonga, Oviedo, Spain

    C. Hardisson

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  1. M. C. Mendoza
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  2. A. J. Gonzalez
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  3. F. J. Mendez
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  4. C. Hardisson
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Mendoza, M.C., Gonzalez, A.J., Mendez, F.J. et al. Plasmid typing of Shigella sonnei epidemic strains and molecular relationship of their R-plasmids. Eur J Epidemiol 4, 158–163 (1988). https://doi.org/10.1007/BF00144744

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  • DOI: https://doi.org/10.1007/BF00144744

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